BPA and low-Se exacerbate apoptosis and mitophagy in chicken pancreatic cells by regulating the PTEN/PI3K/AKT/mTOR pathway

• Published in: Journal of advanced research 2025-01, Vol.67, p.61-69
• Main Authors: Sun, Wenying, Lei, Yutian, Jiang, Zhihui, Wang, Kun, Liu, Huanyi, Xu, Tong
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Apoptosis - drug effects; Benzhydryl Compounds; Bisphenol A; Chickens; Low-Se; Mitophagy; Mitophagy - drug effects; Oxidative Stress - drug effects; Pancreas - drug effects; Pancreas - metabolism; Phenols; Phosphatidylinositol 3-Kinases - metabolism; Proto-Oncogene Proteins c-akt - metabolism; PTEN Phosphohydrolase - metabolism; PTEN/PI3K/AKT/mTOR; Selenium - pharmacology; Signal Transduction - drug effects; TOR Serine-Threonine Kinases - metabolism
• ISSN: 2090-1232; 2090-1224; 2090-1224
• DOI: 10.1016/j.jare.2024.01.029

[Display omitted] •BPA and low-Se induced pancreatic tissue damage, apoptosis, and mitophagy.•PTEN/PI3K/AKT/mTOR pathway was involved in apoptosis and mitophagy induced by BPA and low-Se.•BPA and low-Se induced mitochondrial dysfunction and homeostasis imbalance.•The co-exposure of BPA and low-Se exacerbated pancreatic tissue damage, apoptosis, and mitophagy. Bisphenol A (BPA) is a widespread environmental pollutant which has serious toxic effects on organisms. One of the crucial trace elements is selenium (Se), whose shortage can harm biological tissues and enhance the toxicity of contaminants, in which apoptosis and autophagy are core events. An in vivo model was established to investigate the effects of BPA and low-Se on chicken pancreatic tissue, and identify the possible potential molecular mechanism. A total of 80 1-day-old broiler chickens (Xinghua Chicken Farm, Harbin, China) were stochastically divided into 4 groups (n = 20/group): Control group, BPA group, low-Se group, and low-Se + BPA group. Pancreatic tissue was collected at day 42 to detect changes in markers. First, the data showed that BPA and low-Se exposure gave rose to structural abnormalities in pancreatic tissue, oxidative stress, mitochondrial dysfunction and homeostasis imbalance, apoptosis and mitophagy. In addition, the co-exposure of BPA and low-Se caused the most serious damage to pancreatic tissue. In terms of mechanism, it was found that apoptosis and mitophagy induced by BPA and low-Se were related to the activation of PTEN/PI3K/AKT/mTOR pathway. In summary, the study found that BPA and low-Se exacerbated mitochondria damage, apoptosis and mitophagy by regulating the PTEN/PI3K/AKT/mTOR pathway.