CircCEMIP promotes anoikis-resistance by enhancing protective autophagy in prostate cancer cells

Circular RNAs (circRNAs) are essential participants in the development and progression of various malignant tumors. Previous studies have shown that cell migration-inducing protein (CEMIP) accelerates prostate cancer (PCa) anoikis resistance (AR) by activating autophagy. This study focused on the ef...

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Bibliographic Details
Published in:Journal of experimental & clinical cancer research 2022-06, Vol.41 (1), p.188-188, Article 188
Main Authors: Yu, Ying, Song, Yarong, Cheng, Lulin, Chen, Liang, Liu, Bing, Lu, Dingheng, Li, Xuexiang, Li, Yunxue, Lv, Fang, Xing, Yifei
Format: Article
Language:English
Subjects:
Androgens
Anoikis
Apoptosis
Autophagy
Cancer
Cell adhesion & migration
circ_0004585
Development and progression
Fluorescence
Gene expression
Genetic aspects
Instrument industry
Metastasis
Oncology, Experimental
Prostate
Prostate cancer
Software
TM9SF4
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Summary:Circular RNAs (circRNAs) are essential participants in the development and progression of various malignant tumors. Previous studies have shown that cell migration-inducing protein (CEMIP) accelerates prostate cancer (PCa) anoikis resistance (AR) by activating autophagy. This study focused on the effect of circCEMIP on PCa metastasis. This study gradually revealed the role of circ_0004585 in PCa anoikis resistance via quantitative real-time PCR (qRT-PCR) analysis, western blotting, pull-down assays, and dual fluorescence reporter assays. Functionally, circ_0004585 promoted PCa cells invasion and metastasis both in vitro and in vivo. Mechanistically, circ_0004585 directly interacted with miR-1248 to upregulate target gene expression. Furthermore, target prediction and dual-luciferase reporter assays identified transmembrane 9 superfamily member 4 (TM9SF4) as a potential miR-1248 target. Pathway analysis revealed that TM9SF4 activated autophagy to promote PCa cells anoikis resistance via mTOR phosphorylation. These results demonstrated that circ_0004585 played an oncogenic role during PCa invasion and metastasis by targeting the miR-1248/TM9SF4 axis while providing new insight into therapeutic strategy development for metastatic PCa.
ISSN:1756-9966
0392-9078
1756-9966
DOI:10.1186/s13046-022-02381-7