SIRT3 functions as a tumor suppressor in hepatocellular carcinoma

Hepatocellular carcinoma is one of the leading causes for cancer-related mortality worldwide. SIRT3 may function as either oncogene or tumor suppressor in a panel of cancers; however, the role of SIRT3 in hepatocellular carcinoma remains unclear. In this study, we assayed the expression level of SIR...

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Bibliographic Details
Published in:Tumor biology 2017-03, Vol.39 (3), p.1010428317691178-1010428317691178
Main Authors: Zeng, Xianchun, Wang, Nanzhu, Zhai, Hui, Wang, Rongpin, Wu, Jiahong, Pu, Wei
Format: Article
Language:English
Subjects:
1-Phosphatidylinositol 3-kinase
AKT protein
Biological activity
Carcinoma, Hepatocellular - enzymology
Carcinoma, Hepatocellular - genetics
Carcinoma, Hepatocellular - pathology
Cell growth
Cell Line, Tumor
Cell migration
Cell Movement - physiology
Cell proliferation
Cell Proliferation - physiology
Cloning
Dehydrogenases
Down-Regulation
Gene expression
Hep G2 Cells
Hepatocellular carcinoma
Humans
Immunohistochemistry
Invasiveness
Liver cancer
Liver Neoplasms - enzymology
Liver Neoplasms - genetics
Liver Neoplasms - pathology
Phosphatidylinositol 3-Kinases - metabolism
Polymerase chain reaction
Proto-Oncogene Proteins c-akt - metabolism
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
RNA-directed DNA polymerase
Sirtuin 3 - biosynthesis
Sirtuin 3 - genetics
Tumor suppressor genes
Tumorigenesis
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Summary:Hepatocellular carcinoma is one of the leading causes for cancer-related mortality worldwide. SIRT3 may function as either oncogene or tumor suppressor in a panel of cancers; however, the role of SIRT3 in hepatocellular carcinoma remains unclear. In this study, we assayed the expression level of SIRT3 in hepatocellular carcinoma tissues by quantitative reverse transcriptase-polymerase chain reaction and immunohistochemistry. A loss-of-function approach was used to examine the effects of SIRT3 on biological activity, including cell proliferative activity and invasive potential. The results demonstrated that the expression levels of SIRT3 protein in hepatocellular carcinoma tissues were significantly downregulated compared with those in adjacent non-cancerous tissues. Furthermore, SIRT3 could decrease cell proliferation and inhibit cell migration/invasion in hepatocellular carcinoma cell line. Taken together, these results elucidated the function of SIRT3 in hepatocellular carcinoma development and suggested that SIRT3 might function as tumor suppressor in hepatocellular carcinoma by targeting PI3K/Akt pathway.
ISSN:1010-4283
1423-0380
DOI:10.1177/1010428317691178