Same day sputum smear microscopy with an economical destaining step for the diagnosis of pulmonary tuberculosis

Back ground: Acid fast bacilli (AFB) positivity using modified Ziehl Neelsen (MZN) staining and jar method was studied in sputum samples obtained by spot morning (SM) and same day (SS2) approaches. Methods: Three specimens (spot, second spot and morning) were collected in 252 patients, duplicate sme...

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Bibliographic Details
Published in:Journal of Clinical and Scientific Research 2015-07, Vol.4 (3), p.244-247
Main Authors: Jaya Chandra T, Selvaraj R, Sharma YV
Format: Article
Language:English
Subjects:
modified Ziehl Neelsen staining
Same day approach
Spot morning approach
Online Access:Get full text
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Summary:Back ground: Acid fast bacilli (AFB) positivity using modified Ziehl Neelsen (MZN) staining and jar method was studied in sputum samples obtained by spot morning (SM) and same day (SS2) approaches. Methods: Three specimens (spot, second spot and morning) were collected in 252 patients, duplicate smears were prepared and stained by MZN staining and jar method. Results: Smear positivity was 34 (13.5%) and 33 (13%) for MZN staining and jar method respectively (p>0.99) for the SM approach; and 34 (13.5%) and 32 (12.7%) for MZN staining and jar method (p>0.99) respectively for SS2 approach. Conclusion: Jar method in view of lesser cost and similar diagnostic yielf can be useful for training purposes.
ISSN:2277-5706
2277-8357
DOI:10.15380/2277-5706.JCSR.15.019