Structural and Signaling Events Driving Aspergillus fumigatus -Induced Human Eosinophil Extracellular Trap Release

• Published in: Frontiers in microbiology 2021-02, Vol.12, p.633696-633696
• Main Authors: Barroso, Marina Valente, Gropillo, Isabella, Detoni, Marcella A A, Thompson-Souza, Glaucia A, Muniz, Valdirene S, Vasconcelos, Claudia Regina Isaías, Figueiredo, Rodrigo T, Melo, Rossana C N, Neves, Josiane S
• Format: Article
• Language: English
• Subjects: A. fumigatus; ABPA; allergic bronchopulmonary aspergillosis; eosinophils; extracellular DNA traps; Microbiology
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2021.633696

Eosinophils are granulocytes classically involved in allergic diseases and in the host immune responses to helminths, fungi, bacteria and viruses. The release of extracellular DNA traps by leukocytes is an important mechanism of the innate immune response to pathogens in various infectious conditions, including fungal infections. is an opportunistic fungus responsible for allergic bronchopulmonary aspergillosis (ABPA), a pulmonary disease marked by prominent eosinophilic inflammation. Previously, we demonstrated that isolated human eosinophils release extracellular DNA traps (eosinophil extracellular traps; EETs) when stimulated by . This release occurs through a lytic non-oxidative mechanism that involves CD11b and Syk tyrosine kinase. In this work, we unraveled different intracellular mechanisms that drive the release of extracellular DNA traps by -stimulated eosinophils. Ultrastructurally, we originally observed that -stimulated eosinophils present typical signs of extracellular DNA trap cell death (ETosis) with the nuclei losing both their shape (delobulation) and the euchromatin/heterochromatin distinction, followed by rupture of the nuclear envelope and EETs release. We also found that by targeting class I PI3K, and more specifically PI3Kδ, the release of extracellular DNA traps induced by is inhibited. We also demonstrated that -induced EETs release depends on the Src family, Akt, calcium and p38 MAPK signaling pathways in a process in which fungal viability is dispensable. Interestingly, we showed that -induced EETs release occurs in a mechanism independent of PAD4 histone citrullination. These findings may contribute to a better understanding of the mechanisms that underlie EETs release in response to , which may lead to better knowledge of ABPA pathophysiology and treatment.