A Simplified and Efficient Method for Production of Manganese Ferrite Magnetic Nanoparticles and Their Application in DNA Isolation

A simplified, fast, and effective production method has been developed for the synthesis of manganese ferrite (MnFe O ) magnetic nanoparticles (MNPs). In addition to the wide applicability of MnFe O MNPs, this work also reports their application in DNA isolation for the first time. An ultrasonic-cav...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-01, Vol.24 (3), p.2156
Main Authors: Gerzsenyi, Tímea B, Ilosvai, Ágnes M, Szilágyi, Gergely, Szőri, Milán, Váradi, Csaba, Viskolcz, Béla, Vanyorek, László, Szőri-Dorogházi, Emma
Format: Article
Language:English
Subjects:
Cavitation
Deoxyribonucleic acid
DNA
DNA isolation
DNA, Bacterial
Electrophoresis
Ferric Compounds
ferrite
hydrogen bridge
Magnetic fields
magnetic phase
Magnetite Nanoparticles
Manganese
Manganese Compounds
Nanoparticles
Particle size
Plasmids
Production methods
Sodium Chloride
Temperature
Transmission electron microscopy
Zeta potential
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Summary:A simplified, fast, and effective production method has been developed for the synthesis of manganese ferrite (MnFe O ) magnetic nanoparticles (MNPs). In addition to the wide applicability of MnFe O MNPs, this work also reports their application in DNA isolation for the first time. An ultrasonic-cavitation-assisted combustion method was applied in the synthesis of MnFe O MNPs at different furnace temperatures (573 K, 623 K, 673 K, and 773 K) to optimize the particles' properties. It was shown that MnFe O nanoparticles synthesized at 573 K consist of a spinel phase only with adequate size and zeta potential distributions and superparamagnetic properties. It was also demonstrated that superparamagnetic manganese ferrite nanoparticles bind DNA in buffer with a high NaCl concentration (2.5 M), and the DNA desorbs from the MNPs by decreasing the NaCl concentration of the elution buffer. This resulted in a DNA yield comparable to that of commercial DNA extraction products. Both the DNA concentration measurements and electrophoresis confirmed that a high amount of isolated bacterial plasmid DNA (pDNA) with adequate purity can be extracted with MnFe O (573 K) nanoparticles by applying the DNA extraction method proposed in this article.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24032156