Development of Dot-ELISA and Colloidal Gold Immunochromatographic Strip for Rapid and Super-Sensitive Detection of Plum Pox Virus in Apricot Trees

Plum pox virus (PPV) is a causal agent of the stone fruit tree sharka disease that often causes enormous economic losses. Due to its worldwide distribution and economic importance, rapid and reliable diagnostic technologies are becoming increasingly important for successful management of sharka dise...

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Bibliographic Details
Published in:Viruses 2023-01, Vol.15 (1), p.169
Main Authors: Guo, Mengmeng, Qi, Duo, Dong, Jinxi, Dong, Saiyu, Yang, Xiuling, Qian, Yajuan, Zhou, Xueping, Wu, Jianxiang
Format: Article
Language:English
Subjects:
Antibodies
Antibodies, Monoclonal
apricot tree
colloidal gold immunochromatographic strip
dot-ELISA
Economic importance
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Fruits
Genomes
Gold Colloid
Leaves
Methods
Monoclonal antibodies
monoclonal antibody
Plant Diseases
Plant virus diseases
Plant viruses
Plum pox
Plum Pox Virus
Polyethylene glycol
Polymerase chain reaction
Prunus armeniaca
Quarantine
RT-PCR
Serology
Trees
Viruses
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Summary:Plum pox virus (PPV) is a causal agent of the stone fruit tree sharka disease that often causes enormous economic losses. Due to its worldwide distribution and economic importance, rapid and reliable diagnostic technologies are becoming increasingly important for successful management of sharka disease. In this study, we have produced two super-sensitive and specific anti-PPV monoclonal antibodies (i.e., MAbs 13H4 and 4A11). Using these two MAbs, we have now developed a dot enzyme-linked immunosorbent assay (dot-ELISA) and a colloidal gold immunochromatographic strip (CGICS) assay. These two technologies can be used to quickly and reliably detect PPV. The results of these sensitivity assays confirmed that the dot-ELISA and CGICS assays could detect PPV infection in apricot tree leaf crude extracts diluted up to 1:5120 and 1:6400 ( / ), respectively. Further analyses using field-collected apricot tree leaf samples showed that the detection endpoint of the dot-ELISA was ~26 times above that obtained through RT-PCR, and the CGICS was as sensitive as RT-PCR. This present study is to broaden the knowledge about detection limits of dot-ELISA and CGICS for PPV monitoring. We consider that these newly developed dot-ELISA and CGICS are particularly useful for large scale PPV surveys in fields.
ISSN:1999-4915
1999-4915
DOI:10.3390/v15010169