Tailoring the models of transcription

Molecular biology is a rapidly evolving field that has led to the development of increasingly sophisticated technologies to improve our capacity to study cellular processes in much finer detail. Transcription is the first step in protein expression and the major point of regulation of the components...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2013-04, Vol.14 (4), p.7583-7597
Main Author: Pance, Alena
Format: Article
Language:English
Subjects:
Animals
chimera
Gene Expression Regulation - physiology
Humans
Models, Biological
Molecular Biology - methods
Molecular Biology - trends
promoter
Promoter Regions, Genetic - physiology
reporter gene
Review
transcription
transcription factor
Transcription Factors - metabolism
Transcription, Genetic - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Molecular biology is a rapidly evolving field that has led to the development of increasingly sophisticated technologies to improve our capacity to study cellular processes in much finer detail. Transcription is the first step in protein expression and the major point of regulation of the components that determine the characteristics, fate and functions of cells. The study of transcriptional regulation has been greatly facilitated by the development of reporter genes and transcription factor expression vectors, which have become versatile tools for manipulating promoters, as well as transcription factors in order to examine their function. The understanding of promoter complexity and transcription factor structure offers an insight into the mechanisms of transcriptional control and their impact on cell behaviour. This review focuses on some of the many applications of molecular cut-and-paste tools for the manipulation of promoters and transcription factors leading to the understanding of crucial aspects of transcriptional regulation.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms14047583