SmSPL6 Induces Phenolic Acid Biosynthesis and Affects Root Development in Salvia miltiorrhiza

Salvia miltiorrhiza is a renowned model medicinal plant species for which 15 SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) family genes have been identified; however, the specific functions of SmSPLs have not been well characterized as of yet. For this study, the expression patterns of SmSPL6 were de...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-08, Vol.22 (15), p.7895
Main Authors: Cao, Yao, Chen, Rui, Wang, Wen-Tao, Wang, Dong-Hao, Cao, Xiao-Yan
Format: Article
Language:English
Subjects:
Abscisic acid
Acetic acid
Anthocyanins
Biosynthesis
Enzymes
Genes
Genotype & phenotype
Gibberellic acid
Herbal medicine
Hormones
Indoleacetic acid
Ingredients
Investigations
Jasmonic acid
Phase transitions
Phenolic acids
Phylogenetics
root development
Salvia miltiorrhiza
salvianolic acid B
SPL
transcription factor
Transcription factors
Transgenic plants
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Summary:Salvia miltiorrhiza is a renowned model medicinal plant species for which 15 SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) family genes have been identified; however, the specific functions of SmSPLs have not been well characterized as of yet. For this study, the expression patterns of SmSPL6 were determined through its responses to treatments of exogenous hormones, including indole acetic acid (IAA), gibberellic acid (GA3), methyl jasmonic acid (MeJA), and abscisic acid (ABA). To characterize its functionality, we obtained SmSPL6-ovexpressed transgenic S. miltiorrhiza plants and found that overexpressed SmSPL6 promoted the accumulation of phenolic acids and repressed the biosynthesis of anthocyanin. Meanwhile, the root lengths of the SmSPL6-overexpressed lines were significantly longer than the control; however, both the fresh weights and lateral root numbers decreased. Further investigations indicated that SmSPL6 regulated the biosynthesis of phenolic acid by directly binding to the promoter regions of the enzyme genes Sm4CL9 and SmCYP98A14 and activated their expression. We concluded that SmSPL6 regulates not only the biosynthesis of phenolic acids, but also the development of roots in S. miltiorrhiza.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22157895