Maximum likelihood-based estimation of diffusion coefficient is quick and reliable method for analyzing estradiol actions on surface receptor movements

The rapid effects of estradiol on membrane receptors are in the focus of the estradiol research field, however, the molecular mechanisms of these non-classical estradiol actions are poorly understood. Since the lateral diffusion of membrane receptors is an important indicator of their function, a de...

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Bibliographic Details
Published in:Frontiers in neuroinformatics 2023-03, Vol.17, p.1005936-1005936
Main Authors: Makkai, Geza, Abraham, Istvan M, Barabas, Klaudia, Godo, Soma, Ernszt, David, Kovacs, Tamas, Kovacs, Gergely, Szocs, Szilard, Janosi, Tibor Z
Format: Article
Language:English
Subjects:
17β-Estradiol
Cell membranes
Diffusion coefficient
Labeling
Lateral diffusion
Localization
maximum likelihood
mean square displacement
Methods
Microscopy
MLE
Molecular modelling
Neuroscience
receptor movements
Signal transduction
Software
α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid
α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors
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Summary:The rapid effects of estradiol on membrane receptors are in the focus of the estradiol research field, however, the molecular mechanisms of these non-classical estradiol actions are poorly understood. Since the lateral diffusion of membrane receptors is an important indicator of their function, a deeper understanding of the underlying mechanisms of non-classical estradiol actions can be achieved by investigating receptor dynamics. Diffusion coefficient is a crucial and widely used parameter to characterize the movement of receptors in the cell membrane. The aim of this study was to investigate the differences between maximum likelihood-based estimation (MLE) and mean square displacement (MSD) based calculation of diffusion coefficients. In this work we applied both MSD and MLE to calculate diffusion coefficients. Single particle trajectories were extracted from simulation as well as from α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor tracking in live estradiol-treated differentiated PC12 (dPC12) cells. The comparison of the obtained diffusion coefficients revealed the superiority of MLE over the generally used MSD analysis. Our results suggest the use of the MLE of diffusion coefficients because as it has a better performance, especially for large localization errors or slow receptor movements.
ISSN:1662-5196
1662-5196
DOI:10.3389/fninf.2023.1005936