Microbial Composition of Extracted Dental Alveoli in Dogs with Advanced Periodontitis

• Published in: Microorganisms (Basel) 2024-07, Vol.12 (7), p.1455
• Main Authors: Šakarnytė, Laura, Mockeliūnas, Raimundas, Šiugždinienė, Rita, Merkevičienė, Lina, Virgailis, Marius, Dailidavičienė, Jurgita, Štreimikytė-Mockeliūnė, Žaneta, Ruzauskas, Modestas
• Format: Article
• Language: English
• Subjects: Alveoli; Aminoglycosides; Ampicillin; Animals; antibiotic resistance; Antibiotics; Antimicrobial agents; Antimicrobial resistance; Bacteria; Bacteriology; Bacteroides forsythus; Bone composition; canine; Cefotaxime; Chloramphenicol; Chloromycetin; Ciprofloxacin; Colistin; Dental alveoli; disease prevention; Dogs; Doxycycline; Drug resistance; Etiology; Gene sequencing; Genomes; Gum disease; macrolides; Metagenomics; Microbiology; Microbiota; Microorganisms; Neisseria; One Health initiative; Pasteurella; Pathogenesis; Periodontal disease; Periodontal diseases; Periodontitis; Polymerase chain reaction; Porphyromonas; Porphyromonas cangingivalis; Porphyromonas gulae; Prevotella; Prophylaxis; Soft tissues; species; Streptococcus; Sulfamethoxazole; Taxonomy; Teeth; tissues; traditional bacteriology; Trimethoprim; Veterinary medicine; Veterinary surgeons
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms12071455

Periodontitis is a serious gum infection that damages the soft tissue and destroys the bone supporting the teeth. The aim of the study was to investigate the microbiota using traditional microbiology plating and metagenomic sequencing of extracted tooth alveoli in dogs with severe periodontitis. Isolation of culturable microorganisms was performed as part of bacteriological testing to provide bacteriological diagnosis to veterinary surgeons. Metagenomic sequencing was performed using shotgun sequencing on the Illumina HiSeq system platform. The most prevalent species at sites of periodontal infection detected by metagenomic sequencing were , spp., , , , and . , , and were the most frequently isolated culturable bacteria from infected sites detected by traditional microbiologic methods. Metagenomic data revealed that these three genera accounted for only 1.6% of all microbiota at the sites of infection. Antimicrobial resistance patterns of the isolated bacteria included resistance to ampicillin, doxycycline, sulfamethoxazole-trimethoprim, ciprofloxacin, colistin, cefotaxime, and chloramphenicol. Antimicrobial-resistant genes detected using shotgun sequencing also showed resistance to aminoglycosides and macrolides. Dogs with periodontal infections carry bacteria that can cause bite infections in humans as well as multi-resistant isolates. Therefore, treatment and prophylaxis or periodontal disease of dogs is important from a One Health perspective.