Novel Cryopreservation Approach Providing Off-the-Shelf Availability of Human Multipotent Mesenchymal Stromal Cells for Clinical Applications

Cryopreservation is the only established method to provide long-term storage and fast availability of cellular product for therapeutic applications. The overwhelming majority of cryopreservation media contain toxic concentrations of dimethyl sulfoxide (DMSO) limiting the possibility for the direct a...

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Bibliographic Details
Published in:Stem cells international 2019, Vol.2019 (2019), p.1-11
Main Authors: Petrenko, Yuriy, Petrenko, Alexander, Volkova, Natalia, Mazur, Svitlana, Grischuk, Viktor, Revenko, Olena, Tykhvynska, Olga, Rogulska, Olena, Vasyliev, Roman
Format: Article
Language:English
Subjects:
Anticoagulants
Availability
Biocompatibility
Blood plasma
Blood platelets
Cell culture
Cryopreservation
Damage localization
Dimethyl sulfoxide
Health aspects
Hydrogels
In vivo methods and tests
Mesenchymal stem cells
Mesenchyme
Methods
Morphology
Pretreatment
Stem cells
Stromal cells
Sucrose
Sugar
Thawing
Therapeutic applications
Tissue engineering
Viability
Wound healing
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Summary:Cryopreservation is the only established method to provide long-term storage and fast availability of cellular product for therapeutic applications. The overwhelming majority of cryopreservation media contain toxic concentrations of dimethyl sulfoxide (DMSO) limiting the possibility for the direct administration of cryopreserved cells to the patients. Here, we propose a novel approach for nontoxic xeno-free cryopreservation of human multipotent mesenchymal stromal cells (MSCs) aimed at ensuring high viability, ready-to-use availability, and localized delivery of the cell-based graft into damaged tissues. For MSC cryopreservation, we applied sucrose pretreatment procedure and xeno-free cryoprotective medium containing human platelet-poor blood plasma (PPP), sucrose, and nontoxic concentration of DMSO. Using the combination of PPP, 0.2 M sucrose, and 1% DMSO, the recovery rate of cryopreserved MSCs reached 73% of the values obtained for noncryopreserved cells. Moreover, the presence of PPP in the cryoprotective medium provided the possibility to create a ready-to-use 3D hydrogel for the localized delivery and additional support of MSCs in vivo. In a proof-of-concept study, we assessed the regenerative capacity of cryopreserved MSCs in a full-thickness wound model in mice. The positive impact of MSCs within 3D gel on wound healing rates was confirmed by morphometric and histological examinations. Our results demonstrate the possibility to apply cryopreserved cells immediately after thawing using a cryoprotective medium as the vehicle solution.
ISSN:1687-966X
1687-9678
1687-9678
DOI:10.1155/2019/4150690