Quantitative Detection of ID4 Gene Aberrant Methylation in the Differentiation of Myelodysplastic Syndrome from Aplastic Anemia

Background：The diagnosis of myelodysplastic syndrome （MDS）,especially hypoplastic MDS,and MDS with low blast counts or normal karyotype may be problematic.This study characterized ID4 gene methylation in patients with MDS and aplastic anemia （AA）.Methods：The methylation status ofID4 was analyzed by...

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Published in:Chinese medical journal 2015-08, Vol.128 (15), p.2019-2025
Main Authors: Li, Mian-Yang, Xu, Yuan-Yuan, Kang, Hui-Yuan, Wang, Xin-Rong, Gao, Li, Cen, Jian, Wang, Wei, Wang, Nan, Li, Yong-Hui, Wang, Li-Li, Yu, Li
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Analysis
Anemia
Anemia, Aplastic - genetics
blast
Cancer
Care and treatment
Cell cycle
Child
Chromosomes
Cloning
CpG Islands - genetics
Deoxyribonucleic acid
Diagnosis
Diagnosis
ID4 Gene
Myelodysplastic Syndrome
Methylation
Quantitative
DNA
DNA methylation
DNA Methylation - genetics
Epigenetic inheritance
Epigenetics
Female
Gene expression
Genetic testing
Health aspects
Hematology
Humans
Inhibitor of Differentiation Proteins - genetics
Kinases
Laboratories
Leukemia
Lymphoma
Male
Methylation
Middle Aged
Myelodysplastic syndromes
Myelodysplastic Syndromes - genetics
normal
Original
Polymerase chain reaction
real-time
Tumors
Young Adult
再生障碍性贫血
定量检测
异常
甲基化
综合征
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cited_by cdi_FETCH-LOGICAL-c706a-44e73ab3bf18142104b12e77e6b5e1806c9088ab39e0e43ba3b70cf6b6808b343
cites cdi_FETCH-LOGICAL-c706a-44e73ab3bf18142104b12e77e6b5e1806c9088ab39e0e43ba3b70cf6b6808b343
container_end_page 2025
container_issue 15
container_start_page 2019
container_title Chinese medical journal
container_volume 128
creator Li, Mian-Yang
Xu, Yuan-Yuan
Kang, Hui-Yuan
Wang, Xin-Rong
Gao, Li
Cen, Jian
Wang, Wei
Wang, Nan
Li, Yong-Hui
Wang, Li-Li
Yu, Li
description Background：The diagnosis of myelodysplastic syndrome （MDS）,especially hypoplastic MDS,and MDS with low blast counts or normal karyotype may be problematic.This study characterized ID4 gene methylation in patients with MDS and aplastic anemia （AA）.Methods：The methylation status ofID4 was analyzed by bisulfite sequencing polymerase chain reaction （PCR） and quantitative real-time methylation-specific PCR （MethyLight PCR） in 100 patients with MDS and 31 patients with AA.Results：The MDS group had a higher ID4 gene methylation positivity rate （22.22%） and higher methylation levels （0.21 [0-3.79]） than the AA group （P 〈 0.05）.Furthermore,there were significant differences between the hypoplastic MDS and AA groups,the MDS with low blast count and the AA groups,and the MDS with normal karyotype and the AA groups.The combination of genetic and epigenetic markers was used in much more patients with MDS （62.5% [35/56]） than the use of genetic markers only （51.79% [29/56]）.Conclusions：These results showed that the detection ofID4 methylation positivity rates and levels could be a useful biomarker for MDS diagnosis.
doi_str_mv 10.4103/0366-6999.161351
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Ltd.</rights><rights>Copyright Medknow Publications &amp; Media Pvt. Ltd. Aug 5, 2015</rights><rights>Copyright © Wanfang Data Co. Ltd. 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ID4 Gene; Myelodysplastic Syndrome; Methylation; Quantitative</subject><subject>DNA</subject><subject>DNA methylation</subject><subject>DNA Methylation - genetics</subject><subject>Epigenetic inheritance</subject><subject>Epigenetics</subject><subject>Female</subject><subject>Gene expression</subject><subject>Genetic testing</subject><subject>Health aspects</subject><subject>Hematology</subject><subject>Humans</subject><subject>Inhibitor of Differentiation Proteins - genetics</subject><subject>Kinases</subject><subject>Laboratories</subject><subject>Leukemia</subject><subject>Lymphoma</subject><subject>Male</subject><subject>Methylation</subject><subject>Middle Aged</subject><subject>Myelodysplastic syndromes</subject><subject>Myelodysplastic Syndromes - genetics</subject><subject>normal</subject><subject>Original</subject><subject>Polymerase chain reaction</subject><subject>real-time</subject><subject>Tumors</subject><subject>Young Adult</subject><subject>再生障碍性贫血</subject><subject>定量检测</subject><subject>异常</subject><subject>甲基化</subject><subject>综合征</subject><issn>0366-6999</issn><issn>2542-5641</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptkt1rFDEUxQdRbK2--ySDQvFla74zeRGWVmuhRUR9DpnMnZ2ss0mbzLSsL_7rZrsf7IoMZODmd05ubk5RvMbojGFEPyAqxEQopc6wwJTjJ8Ux4YxMuGD4aXG82z4qXqQ0R4hwLsXz4ogIQiqCyXHx59to_OAGM7h7KC9gADu44MvQllcXrLwED-W0hhgzVd7A0C178wg4Xw5dVri2hQjZwmx1N0voQ7NMt71Jg7Pl96VvYlhA2ea1nG7LUw8LZ14Wz1rTJ3i1-Z8UPz9_-nH-ZXL99fLqfHo9sRIJM2EMJDU1rVtcYUYwYjUmICWImgOukLAKVVUGFCBgtDa0lsi2ohYVqmrK6ElxtfZtgpnr2-gWJi51ME4_FkKcaRNzWz1oELxmlBncVpxJWikkpLGAMGkMsw3JXh_XXrdjvYDG5ttH0x-YHu541-lZuNdMYqm4ygana4MH41vjZ3oexujz9fXvzi7mBGGOOUIig-83J8VwN0Ia9MIlC31vPIQxaSyxUlJUHGf03T_ozhQrwiuiiNyjZibf1Pk25AbtylRPGaE0j5LITJ39h8pfk5_MBg-ty_UDwemeoAPTD10K_biKRDoE0Rq0MaQUod1NDSO9irReZVavMqvXkc6SN_vT3gm2Gc7A-WaaoR8gpl_9-ABRZ_aXDw8HxpM9Y53HrPQ2_tnl7aazLvjZncuvsj1JCC4kEYrSv1ruD5o</recordid><startdate>20150805</startdate><enddate>20150805</enddate><creator>Li, Mian-Yang</creator><creator>Xu, Yuan-Yuan</creator><creator>Kang, Hui-Yuan</creator><creator>Wang, Xin-Rong</creator><creator>Gao, Li</creator><creator>Cen, Jian</creator><creator>Wang, Wei</creator><creator>Wang, Nan</creator><creator>Li, Yong-Hui</creator><creator>Wang, Li-Li</creator><creator>Yu, Li</creator><general>Medknow Publications Pvt Ltd</general><general>Medknow Publications and Media Pvt. 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Xu, Yuan-Yuan ; Kang, Hui-Yuan ; Wang, Xin-Rong ; Gao, Li ; Cen, Jian ; Wang, Wei ; Wang, Nan ; Li, Yong-Hui ; Wang, Li-Li ; Yu, Li</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c706a-44e73ab3bf18142104b12e77e6b5e1806c9088ab39e0e43ba3b70cf6b6808b343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Analysis</topic><topic>Anemia</topic><topic>Anemia, Aplastic - genetics</topic><topic>blast</topic><topic>Cancer</topic><topic>Care and treatment</topic><topic>Cell cycle</topic><topic>Child</topic><topic>Chromosomes</topic><topic>Cloning</topic><topic>CpG Islands - genetics</topic><topic>Deoxyribonucleic acid</topic><topic>Diagnosis</topic><topic>Diagnosis; ID4 Gene; Myelodysplastic Syndrome; Methylation; Quantitative</topic><topic>DNA</topic><topic>DNA methylation</topic><topic>DNA Methylation - genetics</topic><topic>Epigenetic inheritance</topic><topic>Epigenetics</topic><topic>Female</topic><topic>Gene expression</topic><topic>Genetic testing</topic><topic>Health aspects</topic><topic>Hematology</topic><topic>Humans</topic><topic>Inhibitor of Differentiation Proteins - genetics</topic><topic>Kinases</topic><topic>Laboratories</topic><topic>Leukemia</topic><topic>Lymphoma</topic><topic>Male</topic><topic>Methylation</topic><topic>Middle Aged</topic><topic>Myelodysplastic syndromes</topic><topic>Myelodysplastic Syndromes - genetics</topic><topic>normal</topic><topic>Original</topic><topic>Polymerase chain reaction</topic><topic>real-time</topic><topic>Tumors</topic><topic>Young Adult</topic><topic>再生障碍性贫血</topic><topic>定量检测</topic><topic>异常</topic><topic>甲基化</topic><topic>综合征</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Mian-Yang</creatorcontrib><creatorcontrib>Xu, Yuan-Yuan</creatorcontrib><creatorcontrib>Kang, Hui-Yuan</creatorcontrib><creatorcontrib>Wang, Xin-Rong</creatorcontrib><creatorcontrib>Gao, Li</creatorcontrib><creatorcontrib>Cen, Jian</creatorcontrib><creatorcontrib>Wang, Wei</creatorcontrib><creatorcontrib>Wang, Nan</creatorcontrib><creatorcontrib>Li, Yong-Hui</creatorcontrib><creatorcontrib>Wang, Li-Li</creatorcontrib><creatorcontrib>Yu, Li</creatorcontrib><collection>维普_期刊</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>维普中文期刊数据库</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health &amp; 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source HEAL-Link subscriptions: Lippincott Williams & Wilkins; PubMed Central Free; ProQuest - Publicly Available Content Database
subjects Adolescent
Adult
Aged
Aged, 80 and over
Analysis
Anemia
Anemia, Aplastic - genetics
blast
Cancer
Care and treatment
Cell cycle
Child
Chromosomes
Cloning
CpG Islands - genetics
Deoxyribonucleic acid
Diagnosis
Diagnosis
ID4 Gene
Myelodysplastic Syndrome
Methylation
Quantitative
DNA
DNA methylation
DNA Methylation - genetics
Epigenetic inheritance
Epigenetics
Female
Gene expression
Genetic testing
Health aspects
Hematology
Humans
Inhibitor of Differentiation Proteins - genetics
Kinases
Laboratories
Leukemia
Lymphoma
Male
Methylation
Middle Aged
Myelodysplastic syndromes
Myelodysplastic Syndromes - genetics
normal
Original
Polymerase chain reaction
real-time
Tumors
Young Adult
再生障碍性贫血
定量检测
异常
甲基化
综合征
title Quantitative Detection of ID4 Gene Aberrant Methylation in the Differentiation of Myelodysplastic Syndrome from Aplastic Anemia
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