Pharmacological Inhibition of the VCP/Proteasome Axis Rescues Photoreceptor Degeneration in RHOP23H Rat Retinal Explants

Rhodopsin (RHO) misfolding mutations are a common cause of the blinding disease autosomal dominant retinitis pigmentosa (adRP). The most prevalent mutation, RHOP23H, results in its misfolding and retention in the endoplasmic reticulum (ER). Under homeostatic conditions, misfolded proteins are select...

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Bibliographic Details
Published in:Biomolecules (Basel, Switzerland) Switzerland), 2021-10, Vol.11 (10), p.1528
Main Authors: Sen, Merve, Kutsyr, Oksana, Cao, Bowen, Bolz, Sylvia, Arango-Gonzalez, Blanca, Ueffing, Marius
Format: Article
Language:English
Subjects:
Apoptosis
Binding sites
Cell death
Cell survival
Degradation
Endoplasmic reticulum
Explants
Glycoproteins
Insects
Mammals
Mutation
ocular disease
ocular therapy
Photoreceptors
Proteasomes
Protein folding
Protein synthesis
Proteins
Retina
Retinal degeneration
retinal organotypic culture
Retinitis
Retinitis pigmentosa
Rhodopsin
RHOP23H
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Summary:Rhodopsin (RHO) misfolding mutations are a common cause of the blinding disease autosomal dominant retinitis pigmentosa (adRP). The most prevalent mutation, RHOP23H, results in its misfolding and retention in the endoplasmic reticulum (ER). Under homeostatic conditions, misfolded proteins are selectively identified, retained at the ER, and cleared via ER-associated degradation (ERAD). Overload of these degradation processes for a prolonged period leads to imbalanced proteostasis and may eventually result in cell death. ERAD of misfolded proteins, such as RHOP23H, includes the subsequent steps of protein recognition, targeting for ERAD, retrotranslocation, and proteasomal degradation. In the present study, we investigated and compared pharmacological modulation of ERAD at these four different major steps. We show that inhibition of the VCP/proteasome activity favors cell survival and suppresses P23H-mediated retinal degeneration in RHOP23H rat retinal explants. We suggest targeting this activity as a therapeutic approach for patients with currently untreatable adRP.
ISSN:2218-273X
2218-273X
DOI:10.3390/biom11101528