Integration of Transcriptomics and Metabolomics for Pepper ( Capsicum annuum L.) in Response to Heat Stress

• Published in: International journal of molecular sciences 2019-10, Vol.20 (20), p.5042
• Main Authors: Wang, Jing, Lv, Junheng, Liu, Zhoubin, Liu, Yuhua, Song, Jingshuang, Ma, Yanqing, Ou, Lijun, Zhang, Xilu, Liang, Chengliang, Wang, Fei, Juntawong, Niran, Jiao, Chunhai, Chen, Wenchao, Zou, Xuexiao
• Format: Article
• Language: English
• Subjects: Amino Acids - chemistry; Biosynthesis; Capsicum - chemistry; Capsicum - genetics; Capsicum - growth & development; Cultivars; Fatty acids; Flavonoids; Flavonoids - chemistry; Gene expression; Gene Expression Profiling - methods; Gene Expression Regulation, Plant; Genes; glutathione; Heat; Heat resistance; heat shock protein; Heat shock proteins; heat shock transcription factors; Heat stress; Heat-Shock Response; High temperature; Homeostasis; Metabolic Networks and Pathways; Metabolic pathways; Metabolism; Metabolites; metabolome; Metabolomics; Metabolomics - methods; Organic acids; Peppers; Plant Proteins - genetics; Signal transduction; Sugar; Sugars - chemistry; Thermal resistance; Transcription factors; transcriptome; Transcriptomes
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20205042

Heat stress (HS), caused by extremely high temperatures, is one of the most severe forms of abiotic stress in pepper. In the present study, we studied the transcriptome and metabolome of a heat-tolerant cultivar (17CL30) and a heat-sensitive cultivar (05S180) under HS. Briefly, we identified 5754 and 5756 differentially expressed genes (DEGs) in 17CL30 and 05S180, respectively. Moreover, we also identified 94 and 108 differentially accumulated metabolites (DAMs) in 17CL30 and 05S180, respectively. Interestingly, there were many common HS-responsive genes (approximately 30%) in both pepper cultivars, despite the expression patterns of these HS-responsive genes being different in both cultivars. Notably, the expression changes of the most common HS-responsive genes were typically much more significant in 17CL30, which might explain why 17CL30 was more heat tolerant. Similar results were also obtained from metabolome data, especially amino acids, organic acids, flavonoids, and sugars. The changes in numerous genes and metabolites emphasized the complex response mechanisms involved in HS in pepper. Collectively, our study suggested that the glutathione metabolic pathway played a critical role in pepper response to HS and the higher accumulation ability of related genes and metabolites might be one of the primary reasons contributing to the heat resistance.