Assembly and evolutionary analysis of the complete mitochondrial genome of Trichosanthes kirilowii , a traditional Chinese medicinal plant

( . ) is a valuable plant used for both medicinal and edible purposes. It belongs to the family. However, its phylogenetic position and relatives have been difficult to accurately determine due to the lack of mitochondrial genomic information. This limitation has been an obstacle to the potential ap...

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Bibliographic Details
Published in:PeerJ (San Francisco, CA) CA), 2024-07, Vol.12, p.e17747, Article e17747
Main Authors: Jiang, Zhuanzhuan, Chen, Yuhan, Zhang, Xingyu, Meng, Fansong, Chen, Jinli, Cheng, Xu
Format: Article
Language:English
Subjects:
Agricultural Science
Analysis
Evolution, Molecular
Gene transfer
Genes
Genetics
Genome, Mitochondrial - genetics
Genomes
Genomics
Medicinal plants
Medicine, Botanic
Medicine, Chinese Traditional
Medicine, Herbal
Mitochondrial genome
Multi-branched molecule
Phylogeny
Plant Science
Plants, Medicinal - genetics
RNA
RNA editing
Trichosanthes - chemistry
Trichosanthes - genetics
Trichosanthes kirilowii
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Summary:( . ) is a valuable plant used for both medicinal and edible purposes. It belongs to the family. However, its phylogenetic position and relatives have been difficult to accurately determine due to the lack of mitochondrial genomic information. This limitation has been an obstacle to the potential applications of . in various fields. To address this issue, Illumina and Nanopore HiFi sequencing were used to assemble the mitogenome of . into two circular molecules with sizes of 245,700 bp and 107,049 bp, forming a unique multi-branched structure. The mitogenome contains 61 genes, including 38 protein-coding genes (PCGs), 20 tRNAs, and three rRNAs. Within the 38 PCGs of the . mitochondrial genome, 518 potential RNA editing sites were identified. The study also revealed the presence of 15 homologous fragments that span both the chloroplast and mitochondrial genomes. The phylogenetic analysis strongly supports that . belongs to the family and is closely related to Luffa. Collinearity analysis of five mitogenomes shows a high degree of structural variability. Interestingly, four genes, namely 1, FC, FN, and R, played significant roles in the evolution of . through selection pressure analysis. The comparative analysis of the . mitogenome not only sheds light on its functional and structural features but also provides essential information for genetic studies of the genus of .
ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.17747