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Expression and Role of Vitellogenin Genes in Ovarian Development of Zeugodacus cucurbitae
( ) genes encode the major egg yolk protein precursor in arthropods. In this study, four were identified in (Coquillett). Sequence analysis showed that four had the conserved Vg domain. Phylogenetic analysis indicated that four were homologous to the Vgs of Tephritidae insects. The temporal and spat...
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| Published in: | Insects (Basel, Switzerland) Switzerland), 2022-05, Vol.13 (5), p.452 |
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| container_issue | 5 |
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| container_title | Insects (Basel, Switzerland) |
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| creator | Chen, Dong Han, Hong-Liang Li, Wei-Jun Wang, Jin-Jun Wei, Dong |
| description | (
) genes encode the major egg yolk protein precursor in arthropods. In this study, four
were identified in
(Coquillett). Sequence analysis showed that four
had the conserved Vg domain. Phylogenetic analysis indicated that four
were homologous to the Vgs of Tephritidae insects. The temporal and spatial expression patterns of
were analyzed by quantitative real-time polymerase chain reaction (RT-qPCR), and the four
showed high expression levels in female adults, especially in the fat body. The expression of
and
was down-regulated by a low dosage (0.5 μg) of 20-hydroxyecdysone (20E), and
,
, and
were up-regulated by a high dosage (1.0 and 2.0 μg) of 20E. The expression of
and
was up-regulated by 5 μg of juvenile hormone (JH), while all of the
were down-regulated by a low and high dosage of JH. Expression of
was down-regulated after 24 h of starvation and recovered to normal after nutritional supplementation. After micro-injection of the gene-specific double-stranded RNA, the
' expression was significantly suppressed, and ovarian development was delayed in
females. The results indicate that RNA interference of reproduction-related genes is a potential pest control method that works by manipulating female fertility. |
| doi_str_mv | 10.3390/insects13050452 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_e6d03cab5f364a72bc97f5af18b21f74</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_e6d03cab5f364a72bc97f5af18b21f74</doaj_id><sourcerecordid>2671275515</sourcerecordid><originalsourceid>FETCH-LOGICAL-c417t-feb0ce2d223ec9c076cd997d1edee9e447abd04b0f73228a334b3b6ce0eb6b3a3</originalsourceid><addsrcrecordid>eNpdkktrGzEQgEVpaUKSc29loZde3Ogt76VQ8mogEChJob0IPUauzFpypV3T_vvIcRKS6KJB-vQxMxqEPhD8hbEeH8dUwY2VMCwwF_QN2qdYiRnnAr99Fu-ho1qXuC1JKJHz92iPCUmJmqt99Ovs37pArTGnziTf_cgDdDl0P-MIw5AXkGLqLiBB7VpwvTElmtSdwgaGvF5BGrfwb5gW2Rs31c5Nbio2jgYO0btghgpHD_sBuj0_uzn5Pru6vrg8-XY1c5yocRbAYgfUU8rA9Q4r6XzfK0_AA_TAuTLWY25xUIzSuWGMW2alAwxWWmbYAbrceX02S70ucWXKf51N1PcHuSy0KWN0A2iQHjNnrAhMcqOodb0KwgQyt5QExZvr6861nuwKvGv1FTO8kL68SfGPXuSN7gln7F7w-UFQ8t8J6qhXsbrWSZMgT1VTqQhVQhDR0E-v0GWeSmqt2lKYSMo5adTxjnIl11ogPCVDsN5OgX41Be3Fx-c1PPGPf87uAH97sNs</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2670162441</pqid></control><display><type>article</type><title>Expression and Role of Vitellogenin Genes in Ovarian Development of Zeugodacus cucurbitae</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Chen, Dong ; Han, Hong-Liang ; Li, Wei-Jun ; Wang, Jin-Jun ; Wei, Dong</creator><creatorcontrib>Chen, Dong ; Han, Hong-Liang ; Li, Wei-Jun ; Wang, Jin-Jun ; Wei, Dong</creatorcontrib><description>(
) genes encode the major egg yolk protein precursor in arthropods. In this study, four
were identified in
(Coquillett). Sequence analysis showed that four
had the conserved Vg domain. Phylogenetic analysis indicated that four
were homologous to the Vgs of Tephritidae insects. The temporal and spatial expression patterns of
were analyzed by quantitative real-time polymerase chain reaction (RT-qPCR), and the four
showed high expression levels in female adults, especially in the fat body. The expression of
and
was down-regulated by a low dosage (0.5 μg) of 20-hydroxyecdysone (20E), and
,
, and
were up-regulated by a high dosage (1.0 and 2.0 μg) of 20E. The expression of
and
was up-regulated by 5 μg of juvenile hormone (JH), while all of the
were down-regulated by a low and high dosage of JH. Expression of
was down-regulated after 24 h of starvation and recovered to normal after nutritional supplementation. After micro-injection of the gene-specific double-stranded RNA, the
' expression was significantly suppressed, and ovarian development was delayed in
females. The results indicate that RNA interference of reproduction-related genes is a potential pest control method that works by manipulating female fertility.</description><identifier>ISSN: 2075-4450</identifier><identifier>EISSN: 2075-4450</identifier><identifier>DOI: 10.3390/insects13050452</identifier><identifier>PMID: 35621787</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Arthropods ; Conserved sequence ; Control methods ; Dietary supplements ; Dosage ; Double-stranded RNA ; Ecdysone ; Fat body ; Females ; Fertility ; Gene expression ; Genes ; Genomes ; Homology ; hormone regulation ; Hormones ; Insects ; Juvenile hormones ; melon fly ; Nutrient deficiency ; Nutrition ; nutrition stress ; ovarian development ; Ovaries ; Pest control ; Phosphorylation ; Phylogenetics ; Phylogeny ; Polymerase chain reaction ; Precursors ; Proteins ; Reproduction ; Ribonucleic acid ; RNA ; RNA-mediated interference ; RNAi ; Sequence analysis ; Tissues ; Vitellogenin ; Yolk ; Yolk protein ; Zeugodacus cucurbitae</subject><ispartof>Insects (Basel, Switzerland), 2022-05, Vol.13 (5), p.452</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 by the authors. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-feb0ce2d223ec9c076cd997d1edee9e447abd04b0f73228a334b3b6ce0eb6b3a3</citedby><cites>FETCH-LOGICAL-c417t-feb0ce2d223ec9c076cd997d1edee9e447abd04b0f73228a334b3b6ce0eb6b3a3</cites><orcidid>0000-0002-8777-5268 ; 0000-0002-3625-4358</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2670162441/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2670162441?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35621787$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Dong</creatorcontrib><creatorcontrib>Han, Hong-Liang</creatorcontrib><creatorcontrib>Li, Wei-Jun</creatorcontrib><creatorcontrib>Wang, Jin-Jun</creatorcontrib><creatorcontrib>Wei, Dong</creatorcontrib><title>Expression and Role of Vitellogenin Genes in Ovarian Development of Zeugodacus cucurbitae</title><title>Insects (Basel, Switzerland)</title><addtitle>Insects</addtitle><description>(
) genes encode the major egg yolk protein precursor in arthropods. In this study, four
were identified in
(Coquillett). Sequence analysis showed that four
had the conserved Vg domain. Phylogenetic analysis indicated that four
were homologous to the Vgs of Tephritidae insects. The temporal and spatial expression patterns of
were analyzed by quantitative real-time polymerase chain reaction (RT-qPCR), and the four
showed high expression levels in female adults, especially in the fat body. The expression of
and
was down-regulated by a low dosage (0.5 μg) of 20-hydroxyecdysone (20E), and
,
, and
were up-regulated by a high dosage (1.0 and 2.0 μg) of 20E. The expression of
and
was up-regulated by 5 μg of juvenile hormone (JH), while all of the
were down-regulated by a low and high dosage of JH. Expression of
was down-regulated after 24 h of starvation and recovered to normal after nutritional supplementation. After micro-injection of the gene-specific double-stranded RNA, the
' expression was significantly suppressed, and ovarian development was delayed in
females. The results indicate that RNA interference of reproduction-related genes is a potential pest control method that works by manipulating female fertility.</description><subject>Arthropods</subject><subject>Conserved sequence</subject><subject>Control methods</subject><subject>Dietary supplements</subject><subject>Dosage</subject><subject>Double-stranded RNA</subject><subject>Ecdysone</subject><subject>Fat body</subject><subject>Females</subject><subject>Fertility</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genomes</subject><subject>Homology</subject><subject>hormone regulation</subject><subject>Hormones</subject><subject>Insects</subject><subject>Juvenile hormones</subject><subject>melon fly</subject><subject>Nutrient deficiency</subject><subject>Nutrition</subject><subject>nutrition stress</subject><subject>ovarian development</subject><subject>Ovaries</subject><subject>Pest control</subject><subject>Phosphorylation</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Polymerase chain reaction</subject><subject>Precursors</subject><subject>Proteins</subject><subject>Reproduction</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA-mediated interference</subject><subject>RNAi</subject><subject>Sequence analysis</subject><subject>Tissues</subject><subject>Vitellogenin</subject><subject>Yolk</subject><subject>Yolk protein</subject><subject>Zeugodacus cucurbitae</subject><issn>2075-4450</issn><issn>2075-4450</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdkktrGzEQgEVpaUKSc29loZde3Ogt76VQ8mogEChJob0IPUauzFpypV3T_vvIcRKS6KJB-vQxMxqEPhD8hbEeH8dUwY2VMCwwF_QN2qdYiRnnAr99Fu-ho1qXuC1JKJHz92iPCUmJmqt99Ovs37pArTGnziTf_cgDdDl0P-MIw5AXkGLqLiBB7VpwvTElmtSdwgaGvF5BGrfwb5gW2Rs31c5Nbio2jgYO0btghgpHD_sBuj0_uzn5Pru6vrg8-XY1c5yocRbAYgfUU8rA9Q4r6XzfK0_AA_TAuTLWY25xUIzSuWGMW2alAwxWWmbYAbrceX02S70ucWXKf51N1PcHuSy0KWN0A2iQHjNnrAhMcqOodb0KwgQyt5QExZvr6861nuwKvGv1FTO8kL68SfGPXuSN7gln7F7w-UFQ8t8J6qhXsbrWSZMgT1VTqQhVQhDR0E-v0GWeSmqt2lKYSMo5adTxjnIl11ogPCVDsN5OgX41Be3Fx-c1PPGPf87uAH97sNs</recordid><startdate>20220511</startdate><enddate>20220511</enddate><creator>Chen, Dong</creator><creator>Han, Hong-Liang</creator><creator>Li, Wei-Jun</creator><creator>Wang, Jin-Jun</creator><creator>Wei, Dong</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SS</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PATMY</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PYCSY</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-8777-5268</orcidid><orcidid>https://orcid.org/0000-0002-3625-4358</orcidid></search><sort><creationdate>20220511</creationdate><title>Expression and Role of Vitellogenin Genes in Ovarian Development of Zeugodacus cucurbitae</title><author>Chen, Dong ; Han, Hong-Liang ; Li, Wei-Jun ; Wang, Jin-Jun ; Wei, Dong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-feb0ce2d223ec9c076cd997d1edee9e447abd04b0f73228a334b3b6ce0eb6b3a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Arthropods</topic><topic>Conserved sequence</topic><topic>Control methods</topic><topic>Dietary supplements</topic><topic>Dosage</topic><topic>Double-stranded RNA</topic><topic>Ecdysone</topic><topic>Fat body</topic><topic>Females</topic><topic>Fertility</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genomes</topic><topic>Homology</topic><topic>hormone regulation</topic><topic>Hormones</topic><topic>Insects</topic><topic>Juvenile hormones</topic><topic>melon fly</topic><topic>Nutrient deficiency</topic><topic>Nutrition</topic><topic>nutrition stress</topic><topic>ovarian development</topic><topic>Ovaries</topic><topic>Pest control</topic><topic>Phosphorylation</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Polymerase chain reaction</topic><topic>Precursors</topic><topic>Proteins</topic><topic>Reproduction</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA-mediated interference</topic><topic>RNAi</topic><topic>Sequence analysis</topic><topic>Tissues</topic><topic>Vitellogenin</topic><topic>Yolk</topic><topic>Yolk protein</topic><topic>Zeugodacus cucurbitae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Dong</creatorcontrib><creatorcontrib>Han, Hong-Liang</creatorcontrib><creatorcontrib>Li, Wei-Jun</creatorcontrib><creatorcontrib>Wang, Jin-Jun</creatorcontrib><creatorcontrib>Wei, Dong</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>Environmental Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Environmental Science Collection</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Insects (Basel, Switzerland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Dong</au><au>Han, Hong-Liang</au><au>Li, Wei-Jun</au><au>Wang, Jin-Jun</au><au>Wei, Dong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and Role of Vitellogenin Genes in Ovarian Development of Zeugodacus cucurbitae</atitle><jtitle>Insects (Basel, Switzerland)</jtitle><addtitle>Insects</addtitle><date>2022-05-11</date><risdate>2022</risdate><volume>13</volume><issue>5</issue><spage>452</spage><pages>452-</pages><issn>2075-4450</issn><eissn>2075-4450</eissn><abstract>(
) genes encode the major egg yolk protein precursor in arthropods. In this study, four
were identified in
(Coquillett). Sequence analysis showed that four
had the conserved Vg domain. Phylogenetic analysis indicated that four
were homologous to the Vgs of Tephritidae insects. The temporal and spatial expression patterns of
were analyzed by quantitative real-time polymerase chain reaction (RT-qPCR), and the four
showed high expression levels in female adults, especially in the fat body. The expression of
and
was down-regulated by a low dosage (0.5 μg) of 20-hydroxyecdysone (20E), and
,
, and
were up-regulated by a high dosage (1.0 and 2.0 μg) of 20E. The expression of
and
was up-regulated by 5 μg of juvenile hormone (JH), while all of the
were down-regulated by a low and high dosage of JH. Expression of
was down-regulated after 24 h of starvation and recovered to normal after nutritional supplementation. After micro-injection of the gene-specific double-stranded RNA, the
' expression was significantly suppressed, and ovarian development was delayed in
females. The results indicate that RNA interference of reproduction-related genes is a potential pest control method that works by manipulating female fertility.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>35621787</pmid><doi>10.3390/insects13050452</doi><orcidid>https://orcid.org/0000-0002-8777-5268</orcidid><orcidid>https://orcid.org/0000-0002-3625-4358</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Insects (Basel, Switzerland), 2022-05, Vol.13 (5), p.452 |
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| source | Publicly Available Content Database; PubMed Central |
| subjects | Arthropods Conserved sequence Control methods Dietary supplements Dosage Double-stranded RNA Ecdysone Fat body Females Fertility Gene expression Genes Genomes Homology hormone regulation Hormones Insects Juvenile hormones melon fly Nutrient deficiency Nutrition nutrition stress ovarian development Ovaries Pest control Phosphorylation Phylogenetics Phylogeny Polymerase chain reaction Precursors Proteins Reproduction Ribonucleic acid RNA RNA-mediated interference RNAi Sequence analysis Tissues Vitellogenin Yolk Yolk protein Zeugodacus cucurbitae |
| title | Expression and Role of Vitellogenin Genes in Ovarian Development of Zeugodacus cucurbitae |
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