As a biomarker for gastric cancer, circPTPN22 regulates the progression of gastric cancer through the EMT pathway

Gastric cancer (GC) is one of the most common cancers in the world. Due to the lack of specific symptoms, more than 80% of patients are diagnosed as the advanced stage with a high mortality rate, so the early diagnosis of GC is incredibly essential. Circular RNAs (CircRNAs) are a kind of endogenous...

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Bibliographic Details
Published in:Cancer cell international 2021-01, Vol.21 (1), p.44-44, Article 44
Main Authors: Ma, Shuo, Kong, Shan, Gu, Xinliang, Xu, Yanhua, Tao, Mei, Shen, Lei, Shen, Xianjuan, Ju, Shaoqing
Format: Article
Language:English
Subjects:
Biomarker
Biomarkers
Breast cancer
Cell migration
Cell proliferation
circPTPN22
Colorectal cancer
Diagnosis and prognosis
Epithelial-mesenchymal transformation
Gastric cancer
Gel electrophoresis
Medical diagnosis
Medical prognosis
Mesenchyme
Metastases
Metastasis
MicroRNAs
miRNA
Non-coding RNA
Plasma
Plasmids
Primary Research
Reagents
Tumors
Western blotting
Wound healing
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Summary:Gastric cancer (GC) is one of the most common cancers in the world. Due to the lack of specific symptoms, more than 80% of patients are diagnosed as the advanced stage with a high mortality rate, so the early diagnosis of GC is incredibly essential. Circular RNAs (CircRNAs) are a kind of endogenous non-coding RNA with stable structure, the long half-life, and tumor specificity. It can be used as a diagnostic marker for tumors. Using circRNA sequencing technology screened three pairs of GC and adjacent tissues, and circRNAs with significant expression differences were screened out. The circular structure and characteristics of circPTPN22 were determined by RT-qPCR, agarose gel electrophoresis, Sanger sequencing, RNase R, and actinomycin D assays. Cell Counting Kit-8, colony formation, Transwell, Wound healing, tumor formation in mice and western blotting assays were used to detect the effects of circPTPN22 on the proliferation, invasion, migration, tumor growth of GC cells in vitro and protein expression. CircPTPN22 is up-regulated and positively correlated with metastasis in GC tissues, cells, and plasma. RT-qPCR results showed that circPTPN22 had good diagnostic efficacy and could be used to predict the prognosis of GC patients. In vitro and vivo experiments showed that the downregulation of circPTPN22 could inhibit cell proliferation, migration, and invasion through the epithelial-mesenchymal transformation (EMT) pathway. CircPTPN22 may regulate GC progression through the competitive binding of miRNAs. CircPTPN22 can be used as a potential diagnostic and prognostic marker for GC and can inhibit cell proliferation and metastasis through the competitive binding of miRNA to inhibit the EMT pathway.
ISSN:1475-2867
1475-2867
DOI:10.1186/s12935-020-01701-1