The Calcineurin-Binding, Activity-Dependent Splice Variant Dynamin1xb Is Highly Enriched in Synapses in Various Regions of the Central Nervous System

In the present study, we generated and characterized a splice site-specific monoclonal antibody that selectively detects the calcineurin-binding dynamin1 splice variant dynamin1xb. Calcineurin is a Ca -regulated phosphatase that enhances dynamin1 activity and is an important Ca -sensing mediator of...

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Bibliographic Details
Published in:Frontiers in molecular neuroscience 2017-07, Vol.10, p.230-230
Main Authors: Eich, Marie-Lisa, Dembla, Ekta, Wahl, Silke, Dembla, Mayur, Schwarz, Karin, Schmitz, Frank
Format: Article
Language:English
Subjects:
Alternative splicing
Calcineurin
Calcium
Central nervous system
darkness-induced synaptic recruitment of dynamin1xb
dynamin1xb
Homeostatic plasticity
Monoclonal antibodies
Neuroscience
Phosphorylation
Proteins
Retina
splice variant
synapse
Synaptic plasticity
Synaptic ribbons
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Summary:In the present study, we generated and characterized a splice site-specific monoclonal antibody that selectively detects the calcineurin-binding dynamin1 splice variant dynamin1xb. Calcineurin is a Ca -regulated phosphatase that enhances dynamin1 activity and is an important Ca -sensing mediator of homeostatic synaptic plasticity in neurons. Using this dynamin1xb-specific antibody, we found dynamin1xb highly enriched in synapses of all analyzed brain regions. In photoreceptor ribbon synapses, dynamin1xb was enriched in close vicinity to the synaptic ribbon in a manner indicative of a peri-active zone immunolabeling. Interestingly, in dark-adapted mice we observed an enhanced and selective enrichment of dynamin1xb in both synaptic layers of the retina in comparison to light-adapted mice. This could be due to an illumination-dependent recruitment of dynamin1xb to retinal synapses and/or due to a darkness-induced increase of dynamin1xb biosynthesis. These latter findings indicate that dynamin1xb is part of a versatile and highly adjustable, activity-regulated endocytic synaptic machinery.
ISSN:1662-5099
1662-5099
DOI:10.3389/fnmol.2017.00230