Genetic diversity and seroprevalence of Toxoplasma gondii in COVID‑19 patients; a first case-control study in Iran

• Published in: BMC infectious diseases 2024-01, Vol.24 (1), p.42-42, Article 42
• Main Authors: Hasanzadeh, Mehdi, Ahmadpour, Ehsan, Mahami-Oskouei, Mahmoud, Musavi, Saeed, Parsaei, Mahdi, Sarafraz, Nazila, Spotin, Adel
• Format: Article
• Language: English
• Subjects: Adult; Amplification; Analysis; Animals; Antibodies; Antibodies, Protozoan; Asymptomatic; Biological diversity; Case-Control Studies; Cats; CD4 antigen; COVID-19; COVID-19 - epidemiology; COVID‑19 patients; Disease; Enzyme-linked immunosorbent assay; Ethylenediaminetetraacetic acid; Female; Gene frequency; Genes; Genetic diversity; Genetic Variation; Health aspects; Humans; Immunoglobulin G; Immunoglobulin M; Infection; Iran - epidemiology; Lymphocytes; Lymphocytes T; Medical research; Medicine, Experimental; Middle Aged; Minority & ethnic groups; Mortality; Northwest Iran; Phylogenetics; Phylogeny; Pregnancy; Pregnant women; Prevention; Protozoa; Risk Factors; Sequencing; Seroepidemiologic Studies; Serology; Severe acute respiratory syndrome coronavirus 2; Streptococcus infections; T cells; Thermal cycling; Toxoplasma; Toxoplasma - genetics; Toxoplasma gondii; Toxoplasmosis; Toxoplasmosis - diagnosis; Viral diseases; Young Adult
• ISSN: 1471-2334; 1471-2334
• DOI: 10.1186/s12879-023-08964-9

Toxoplasmosis is a serious or life-threatening disease in immunosuppressed patients and pregnant women. This study examined the likely association between Toxoplasma gondii infection and COVID-19 patients with moderate illness. Seventy blood samples were collected from patients at the Health Reference Laboratory of Tabriz, Northwest Iran from April 2021 to September 2021. In addition, 70 healthy subjects of the same age (37 ± 15 years) and sex distribution were ethnically matched. Sera samples were examined for the detection of anti-Toxoplasma antibodies using ELISA. Nested-PCR targets were amplified based on the B1 and GRA6 genes. GRA6 amplicons were subjected to sequencing and phylogenetic analysis. The seroprevalence of toxoplasmosis based on IgG titer was 35.7% in the COVID‑19 patients and 27.1% in the control group, representing not to be associated with the Toxoplasma seropositivity in COVID‑19 patients (P = 0.18) compared to healthy subjects. Anti-T. gondii IgM was not found in any of the patients and healthy individuals. According to PCR amplification of the B1 and GRA6 genes, the frequency of T. gondii in COVID-19 patients was 14.2% (10/70). However, no T. gondii infection was detected in the healthy group. The CD4 T cell count was relatively lower in toxoplasmosis-infected patients (430-450 cells/mm3) than in control group (500-1500 cells/mm3). High genetic diversity (Hd: 0.710) of the type I strain of T. gondii was characterized in the patients. Present results showed that consumption of raw vegetables and close contact with stray cats can increase the transmission of T. gondii to COVID-19 patients (P