Microbial Metabolite Urolithin B Inhibits Recombinant Human Monoamine Oxidase A Enzyme

Urolithins are gut microbial metabolites derived from ellagitannins (ET) and ellagic acid (EA), and shown to exhibit anticancer, anti-inflammatory, anti-microbial, anti-glycative and anti-oxidant activities. Similarly, the parent molecules, ET and EA are reported for their neuroprotection and antide...

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Bibliographic Details
Published in:Metabolites 2020-06, Vol.10 (6), p.258
Main Authors: Singh, Rajbir, Chandrashekharappa, Sandeep, Vemula, Praveen Kumar, Haribabu, Bodduluri, Jala, Venkatakrishna Rao
Format: Article
Language:English
Subjects:
Amine oxidase (flavin-containing)
Binding sites
Bioavailability
Brain
Communication
Dopamine
Ellagic acid
Enzyme kinetics
Inflammation
Intestinal microflora
Mental depression
Metabolites
microbial metabolites
Microbiota
monoamine oxidase
Monoamines
Movement disorders
Neurodegenerative diseases
Neurological diseases
Neuroprotection
Neurotransmitters
Oxidants
Parkinson's disease
urolithins
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Summary:Urolithins are gut microbial metabolites derived from ellagitannins (ET) and ellagic acid (EA), and shown to exhibit anticancer, anti-inflammatory, anti-microbial, anti-glycative and anti-oxidant activities. Similarly, the parent molecules, ET and EA are reported for their neuroprotection and antidepressant activities. Due to the poor bioavailability of ET and EA, the in vivo functional activities cannot be attributed exclusively to these compounds. Elevated monoamine oxidase (MAO) activities are responsible for the inactivation of monoamine neurotransmitters in neurological disorders, such as depression and Parkinson’s disease. In this study, we examined the inhibitory effects of urolithins (A, B and C) and EA on MAO activity using recombinant human MAO-A and MAO-B enzymes. Urolithin B was found to be a better MAO-A enzyme inhibitor among the tested urolithins and EA with an IC50 value of 0.88 µM, and displaying a mixed mode of inhibition. However, all tested compounds exhibited higher IC50 (>100 µM) for MAO-B enzyme.
ISSN:2218-1989
2218-1989
DOI:10.3390/metabo10060258