MALDI mass spectrometry imaging for the simultaneous location of resveratrol, pterostilbene and viniferins on grapevine leaves

To investigate the in-situ response to a stress, grapevine leaves have been subjected to mass spectrometry imaging (MSI) experiments. The Matrix Assisted Laser Desorption/Ionisation (MALDI) approach using different matrices has been evaluated. Among all the tested matrices, the 2,5-dihydroxybenzoic...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2014-07, Vol.19 (7), p.10587-10600
Main Authors: Becker, Loïc, Carré, Vincent, Poutaraud, Anne, Merdinoglu, Didier, Chaimbault, Patrick
Format: Article
Language:English
Subjects:
Condensed Matter
Experiments
Fungi
grapevine
Infections
Lasers
Leaves
MALDI
Mass spectrometry
mass spectrometry imaging
Materials Science
Metabolites
Oomycetes - microbiology
Pathogens
Physics
Plant Diseases
Plant Leaves - metabolism
Plant Leaves - microbiology
Plasmopara viticola
pterostilbene
Resveratrol
Scientific imaging
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
stilbene phytoalexin
Stilbenes - metabolism
viniferin
Vitis - metabolism
Vitis - microbiology
Vitis vinifera
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Summary:To investigate the in-situ response to a stress, grapevine leaves have been subjected to mass spectrometry imaging (MSI) experiments. The Matrix Assisted Laser Desorption/Ionisation (MALDI) approach using different matrices has been evaluated. Among all the tested matrices, the 2,5-dihydroxybenzoic acid (DHB) was found to be the most efficient matrix allowing a broader range of detected stilbene phytoalexins. Resveratrol, but also more toxic compounds against fungi such as pterostilbene and viniferins, were identified and mapped. Their spatial distributions on grapevine leaves irradiated by UV show their specific colocation around the veins. Moreover, MALDI MSI reveals that resveratrol (and piceids) and viniferins are not specifically located on the same area when leaves are infected by Plasmopara viticola. Results obtained by MALDI mass spectrometry imaging demonstrate that this technique would be essential to improve the level of knowledge concerning the role of the stilbene phytoalexins involved in a stress event.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules190710587