Dihydromyricetin Enhances Exercise-Induced GLP-1 Elevation through Stimulating cAMP and Inhibiting DPP-4

The purpose of this study was to examine whether endogenous GLP-1 (glucagon-like peptide-1) could respond to exercise training in mice, as well as whether dihydromyricetin (DHM) supplementation could enhance GLP-1 levels in response to exercise training. After 2 weeks of exercise intervention, we fo...

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Bibliographic Details
Published in:Nutrients 2022-11, Vol.14 (21), p.4583
Main Authors: Wu, Luting, Zhou, Min, Xie, Yingquan, Lang, Hedong, Li, Tianyou, Yi, Long, Zhang, Qianyong, Mi, Mantian
Format: Article
Language:English
Subjects:
Antibiotics
Antibodies
Appetite
Bioflavonoids
cAMP
Cyclic AMP
Dietary supplements
dihydromyricetin
DPP-4
Exercise
Experiments
Feces
Fitness training programs
Flavones
Flavonoids
Gene expression
GLP-1
Glucagon
Glucagon-like peptide 1
gut microbiota
Health aspects
Intestinal microflora
Laboratory animals
Medical research
Microbiota
Peptidase
Peptidases
Peptide hormones
Physical training
Physiological aspects
Secretion
Transplantation
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Summary:The purpose of this study was to examine whether endogenous GLP-1 (glucagon-like peptide-1) could respond to exercise training in mice, as well as whether dihydromyricetin (DHM) supplementation could enhance GLP-1 levels in response to exercise training. After 2 weeks of exercise intervention, we found that GLP-1 levels were significantly elevated. A reshaped gut microbiota was identified following exercise, as evidenced by the increased abundance of Bifidobacterium, Lactococcus, and Alistipes genus, which are involved in the production of short-chain fatty acids (SCFAs). Antibiotic treatment negated exercise-induced GLP-1 secretion, which could be reversed with gut microbiota transplantation. Additionally, the combined intervention (DHM and exercise) was modeled in mice. Surprisingly, the combined intervention resulted in higher GLP-1 levels than the exercise intervention alone. In exercised mice supplemented with DHM, the gut microbiota composition changed as well, while the amount of SCFAs was unchanged in the stools. Additionally, DHM treatment induced intracellular cAMP in vitro and down-regulated the gene and protein expression of dipeptidyl peptidase-4 (DPP-4) both in vivo and in vitro. Collectively, the auxo-action of exercise on GLP-1 secretion is associated with the gut-microbiota-SCFAs axis. Moreover, our findings suggest that DHM interacts synergistically with exercise to enhance GLP-1 levels by stimulating cAMP and inhibiting DPP-4.
ISSN:2072-6643
2072-6643
DOI:10.3390/nu14214583