Functional Analysis of Pheromone Biosynthesis Activating Neuropeptide Receptor Isoforms in Maruca vitrata

• Published in: Cells (Basel, Switzerland) Switzerland), 2023-05, Vol.12 (10), p.1410
• Main Authors: Cha, Wook Hyun, Kim, Boyun, Lee, Dae-Weon
• Format: Article
• Language: English
• Subjects: Abdomen; Amino Acid Sequence; Animals; Biosynthesis; Butterflies & moths; C-Terminus; Calcium influx; Cell lines; Cell membranes; Cloning; Cockroaches; confocal microscopy; Developmental stages; Epithelial cells; Females; G protein-coupled receptors; Gas chromatography; Glands; HeLa Cells; Humans; Isoforms; Maruca vitrata; Mating behavior; Moths - genetics; Neuropeptides; PBAN receptor; Pheromone biosynthesis-activating neuropeptide; Pheromone gland; Pheromones; Phylogenetics; Protein Isoforms - metabolism; Proteins; Receptors, Neuropeptide - genetics; Receptors, Neuropeptide - metabolism; RNA interference; RNA-mediated interference; Sex Attractants - metabolism; Sex pheromone; Signal transduction
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells12101410

Insect sex pheromones are volatile chemicals that induce mating behavior between conspecific individuals. In moths, sex pheromone biosynthesis is initiated when pheromone biosynthesis-activating neuropeptide (PBAN) synthesized in the suboesophageal ganglion binds to its receptor on the epithelial cell membrane of the pheromone gland. To investigate the function of PBAN receptor (PBANR), we identified two PBANR isoforms, MviPBANR-B and MviPBANR-C, in the pheromone glands of . These two genes belong to G protein-coupled receptors (GPCRs) and have differences in the C-terminus but share a 7-transmembrane region and GPCR family 1 signature. These isoforms were expressed in all developmental stages and adult tissues. MviPBANR-C had the highest expression level in pheromone glands among the examined tissues. Through in vitro heterologous expression in HeLa cell lines, only MviPBANR-C-transfected cells responded to MviPBAN (≥5 µM MviPBAN), inducing Ca influx. Sex pheromone production and mating behavior were investigated using gas chromatography and a bioassay after MviPBANR-C suppression by RNA interference, which resulted in the major sex pheromone component, E10E12-16:Ald, being quantitatively reduced compared to the control, thereby decreasing the mating rate. Our findings indicate that MviPBANR-C is involved in the signal transduction of sex pheromone biosynthesis in and that the C-terminal tail plays an important role in its function.