Comparative transcriptomics of choroid plexus in Alzheimer's disease, frontotemporal dementia and Huntington's disease: implications for CSF homeostasis

• Published in: Fluids and barriers of the CNS 2018-05, Vol.15 (1), p.18-18, Article 18
• Main Authors: Stopa, Edward G, Tanis, Keith Q, Miller, Miles C, Nikonova, Elena V, Podtelezhnikov, Alexei A, Finney, Eva M, Stone, David J, Camargo, Luiz M, Parker, Lisan, Verma, Ajay, Baird, Andrew, Donahue, John E, Torabi, Tara, Eliceiri, Brian P, Silverberg, Gerald D, Johanson, Conrad E
• Format: Article
• Language: English
• Subjects: Adult; Advertising executives; Aged; Aged, 80 and over; Alzheimer Disease - metabolism; Alzheimer's disease; Blood–CSF barrier inflammatome; Brain research; Cadherin-mediated adhesion; Cadherins; Cell adhesion; Cerebrospinal fluid; Choroid plexus; Choroid Plexus - metabolism; Choroid plexus transcriptome; Comparative analysis; Dementia; Dementia disorders; Disease; DNA microarrays; Female; Fluids; Frontotemporal dementia; Frontotemporal Dementia - metabolism; Gene Expression; Genes; Genetic translation; Homeostasis; Homeostasis - physiology; Homocysteine; Humans; Huntington Disease - metabolism; Huntingtons disease; Immune response; Interferon; Interleukins; Janus kinase/signal transducers and activators of transcription (JAK-STAT); Male; Metabolites; Methionine; Microarray Analysis; Middle Aged; Neurodegeneration; Neurodegenerative diseases; Neuroimmune CSF regulation; Peroxisome-proliferator-activated receptor (PPAR); Proteins; Ribonucleic acid; RNA; TOR protein; Transcription; Transcriptome; Vascular endothelial growth factor; Ventricle
• ISSN: 2045-8118; 2045-8118
• DOI: 10.1186/s12987-018-0102-9

In Alzheimer's disease, there are striking changes in CSF composition that relate to altered choroid plexus (CP) function. Studying CP tissue gene expression at the blood-cerebrospinal fluid barrier could provide further insight into the epithelial and stromal responses to neurodegenerative disease states. Transcriptome-wide Affymetrix microarrays were used to determine disease-related changes in gene expression in human CP. RNA from post-mortem samples of the entire lateral ventricular choroid plexus was extracted from 6 healthy controls (Ctrl), 7 patients with advanced (Braak and Braak stage III-VI) Alzheimer's disease (AD), 4 with frontotemporal dementia (FTD) and 3 with Huntington's disease (HuD). Statistics and agglomerative clustering were accomplished with MathWorks, MatLab; and gene set annotations by comparing input sets to GeneGo ( http://www.genego.com ) and Ingenuity ( http://www.ingenuity.com ) pathway sets. Bonferroni-corrected hypergeometric p-values of