Effect of glutamine supplementation on left colon healing in rats with extrahepatic biliary obstruction

• Published in: Acta cirúrgica brasileira 2015-01, Vol.30 (1), p.73-79
• Main Authors: Alecrim, Hugo Muscelli, Duarte, Sérgio Andurte Carvalho, Amaral, Maria Eduarda Bonavides, Diógenis, Filipe, Carneiro, Fabiana Pirani, Sousa, João Batista de
• Format: Article
• Language: English
• Subjects: Bilirubin; Colon; Colorectal Surgery; Glutamine; Jaundice; Rats; Wound Healing
• ISSN: 0102-8650; 0102-8650
• DOI: 10.1590/S0102-86502015001000010

PURPOSE:To evaluate the influence of glutamine and obstructive jaundice on left colon healing in rats.METHODS: Sixteen male rats were allocated across four groups: LG - Common bile duct ligation followed by colotomy and bowel suture on postoperative day 7. Supplementation with glutamine 2% from day 4 after duct ligation until euthanasia. L - Common bile duct ligation followed by colotomy and bowel suture on postoperative day 7. No glutamine supplementation. M - Common bile duct manipulation followed by colotomy and bowel suture on postoperative day 7. No glutamine supplementation. MG - Common bile duct manipulation followed by colotomy and bowel suture on postoperative day 7. Supplementation with glutamine 2% from day 4 after duct manipulation until euthanasia. On the day of euthanasia, bursting pressure of the sutured bowel segment was measured and samples were collected for histopathological analysis.RESULTS:There were no differences in bursting pressure among groups : LG vs. M (110 ± 28 vs. 173 ± 12; p = 0.08). Groups L and MG were not different from group M (156 ± 12 and 118 ± 22. Glutamine supplementation was associated with less edema, polymorphonuclear lymphocyte infiltration, bacterial colonies, and abscess formation, as well as with increased collagen formation.CONCLUSION: Obstructive jaundice had no negative effect and glutamine supplementation had no positive effect on colonic scar strength in rats.