Characterization of Bacillus velezensis UTB96, Demonstrating Improved Lipopeptide Production Compared to the Strain B. velezensis FZB42

Bacillus strains can produce various lipopeptides, known for their antifungal properties. This makes them attractive metabolites for applications in agriculture. Therefore, identification of productive wild-type strains is essential for the development of biopesticides. Bacillus velezensis FZB42 is...

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Published in:Microorganisms (Basel) 2022-11, Vol.10 (11), p.2225
Main Authors: Vahidinasab, Maliheh, Adiek, Isabel, Hosseini, Behnoush, Akintayo, Stephen Olusanmi, Abrishamchi, Bahar, Pfannstiel, Jens, Henkel, Marius, Lilge, Lars, Voegele, Ralf T., Hausmann, Rudolf
Format: Article
Language:English
Subjects:
Agriculture
Alanine
Amino acids
Antifungal activity
bacillomycin D
Bacillus
Bacillus velezensis
Batch culture
Biological control
Bioreactors
Biosynthesis
Fatty acids
fengycin
Fungal diseases
Fungi
Fungicides
Ions
Isoleucine
Iturin A
Leucine
lipopeptide
Lipopeptides
Lysine
Metabolites
Microorganisms
Pathogens
Peptides
Pesticides
Plant resistance
Soybeans
Supplements
Surfactin
Valine
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Summary:Bacillus strains can produce various lipopeptides, known for their antifungal properties. This makes them attractive metabolites for applications in agriculture. Therefore, identification of productive wild-type strains is essential for the development of biopesticides. Bacillus velezensis FZB42 is a well-established strain for biocontrol of plant pathogens in agriculture. Here, we characterized an alternative strain, B. velezensis UTB96, that can produce higher amounts of all three major lipopeptide families, namely surfactin, fengycin, and iturin. UTB96 produces iturin A. Furthermore, UTB96 showed superior antifungal activity towards the soybean fungal pathogen Diaporthe longicolla compared to FZB42. Moreover, the additional provision of different amino acids for lipopeptide production in UTB96 was investigated. Lysine and alanine had stimulatory effects on the production of all three lipopeptide families, while supplementation of leucine, valine and isoleucine decreased the lipopeptide bioproduction. Using a 45-litre bioreactor system for upscaling in batch culture, lipopeptide titers of about 140 mg/L surfactin, 620 mg/L iturin A, and 45 mg/L fengycin were achieved. In conclusion, it becomes clear that B. velezensis UTB96 is a promising strain for further research application in the field of agricultural biological controls of fungal diseases.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms10112225