Draft genome sequence of Acidithiobacillus thiooxidans CLST isolated from the acidic hypersaline Gorbea salt flat in northern Chile

• Published in: Standards in genomic sciences 2017-12, Vol.12 (1), p.84-8, Article 84
• Main Authors: Quatrini, Raquel, Escudero, Lorena V, Moya-Beltrán, Ana, Galleguillos, Pedro A, Issotta, Francisco, Acosta, Mauricio, Cárdenas, Juan Pablo, Nuñez, Harold, Salinas, Karina, Holmes, David S, Demergasso, Cecilia
• Format: Article
• Language: English
• Subjects: Acidithiobacillaceae; Analysis; Bioleaching; DNA sequencing; Extended Genome Report; Flexible gene complement; Genetic aspects; Halotolerance; Nucleotide sequencing; Osmotolerance; Physiological aspects; Salt pans (Geology); Sulfur oxidization; Sulfur-oxidizing bacteria
• ISSN: 1944-3277; 1944-3277
• DOI: 10.1186/s40793-017-0305-8

10.1601/nm.2199 CLST is an extremely acidophilic gamma-proteobacteria that was isolated from the Gorbea salt flat, an acidic hypersaline environment in northern Chile. This kind of environment is considered a terrestrial analog of ancient Martian terrains and a source of new material for biotechnological applications. 10.1601/nm.2199 plays a key role in industrial bioleaching; it has the capacity of generating and maintaining acidic conditions by producing sulfuric acid and it can also remove sulfur layers from the surface of minerals, which are detrimental for their dissolution. CLST is a strain of 10.1601/nm.2199 able to tolerate moderate chloride concentrations (up to 15 g L Cl ), a feature that is quite unusual in extreme acidophilic microorganisms. Basic microbiological features and genomic properties of this biotechnologically relevant strain are described in this work. The 3,974,949 bp draft genome is arranged into 40 scaffolds of 389 contigs containing 3866 protein-coding genes and 75 RNAs encoding genes. This is the first draft genome of a halotolerant 10.1601/nm.2199 strain. The release of the genome sequence of this strain improves representation of these extreme acidophilic Gram negative bacteria in public databases and strengthens the framework for further investigation of the physiological diversity and ecological function of 10.1601/nm.2199 populations.