Inhibitory Effect of Adsorption of Streptococcus mutans onto Scallop-Derived Hydroxyapatite

Hydroxyapatite adsorbs various substances, but little is known about the effects on oral bacteria of adsorption onto hydroxyapatite derived from scallop shells. In the present study, we analyzed the effects of adsorption of onto scallop-derived hydroxyapatite. When scallop-derived hydroxyapatite was...

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Published in:International journal of molecular sciences 2023-07, Vol.24 (14), p.11371
Main Authors: Usuda, Momoko, Kametani, Mariko, Hamada, Masakazu, Suehiro, Yuto, Matayoshi, Saaya, Okawa, Rena, Naka, Shuhei, Matsumoto-Nakano, Michiyo, Akitomo, Tatsuya, Mitsuhata, Chieko, Koumoto, Kazuya, Kawauchi, Keiko, Nishikata, Takahito, Yagi, Masatoshi, Mizoguchi, Toshiro, Fujikawa, Koki, Taniguchi, Taizo, Nakano, Kazuhiko, Nomura, Ryota
Format: Article
Language:English
Subjects:
Adsorption
Amino acids
Antibiotics
Bacteria
bacterial growth
Biocompatibility
Biofilms
Biosynthesis
Bones
Dental caries
Dental implants
Gene expression
Hydroxyapatite
Metabolism
Morphology
Ontology
Principal components analysis
Proteins
RNA sequence
scallop-derived hydroxyapatite
Shells
Streptococcus infections
Streptococcus mutans
Toothpaste
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Summary:Hydroxyapatite adsorbs various substances, but little is known about the effects on oral bacteria of adsorption onto hydroxyapatite derived from scallop shells. In the present study, we analyzed the effects of adsorption of onto scallop-derived hydroxyapatite. When scallop-derived hydroxyapatite was mixed with , a high proportion of the bacterial cells adsorbed onto the hydroxyapatite in a time-dependent manner. An RNA sequencing analysis of adsorbed onto hydroxyapatite showed that the upregulation of genes resulted in abnormalities in pathways involved in glycogen and histidine metabolism and biosynthesis compared with cells in the absence of hydroxyapatite. adsorbed onto hydroxyapatite was not killed, but the growth of the bacteria was inhibited. Electron microscopy showed morphological changes in cells adsorbed onto hydroxyapatite. Our results suggest that hydroxyapatite derived from scallop shells showed a high adsorption ability for . This hydroxyapatite also caused changes in gene expression related to the metabolic and biosynthetic processes, including the glycogen and histidine of , which may result in a morphological change in the surface layer and the inhibition of the growth of the bacteria.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms241411371