Unleashing the Influence of cAMP Receptor Protein: The Master Switch of Bacteriocin Export in Pectobacterium carotovorum subsp. carotovorum

( ) is a Gram-negative phytopathogenic bacterium that produces carocin, a low-molecular-weight bacteriocin that can kill related strains in response to factors in the environment such as UV exposure or nutritional deficiency. The function of the catabolite activator protein (CAP), also known as the...

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Bibliographic Details
Published in:International journal of molecular sciences 2023-06, Vol.24 (11), p.9752
Main Authors: Chang, Chung-Pei, Lagitnay, Ruchi Briam James Sersenia, Li, Tzu-Rong, Lai, Wei-Ting, Derilo, Reymund Calanga, Chuang, Duen-Yau
Format: Article
Language:English
Subjects:
Bacteria
Bacterial infections
Bacteriocins
cAMP receptor protein
Catabolite activator protein
Cyclic AMP
Cyclic AMP receptor protein
E coli
Flagella
flagellar-type III secretion
Gene expression
Genes
In vivo methods and tests
low-molecular-weight bacteriocin
Metabolism
Nucleotide sequence
Nutrient deficiency
Pectobacterium carotovorum
Pectobacterium carotovorum subsp. carotovorum
Polymerase chain reaction
Proteins
Signal transduction
Translation initiation
Virulence
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Summary:( ) is a Gram-negative phytopathogenic bacterium that produces carocin, a low-molecular-weight bacteriocin that can kill related strains in response to factors in the environment such as UV exposure or nutritional deficiency. The function of the catabolite activator protein (CAP), also known as the cyclic AMP receptor protein (CRP), as a regulator of carocin synthesis was examined. The gene was knocked out as part of the investigation, and the outcomes were assessed both in vivo and in vitro. Analysis of the DNA sequence upstream of the translation initiation site of carocin S3 revealed two putative binding sites for CRP that were confirmed using a biotinylated probe pull-down experiment. This study revealed that the deletion of inhibited genes involved in extracellular bacteriocin export via the flagellar type III secretion system and impacted the production of many low-molecular-weight bacteriocins. The biotinylated probe pull-down test demonstrated that when UV induction was missing, CRP preferentially attached to one of the two CAP sites while binding to both when UV induction was present. In conclusion, our research aimed to simulate the signal transduction system that controls the expression of the carocin gene in response to UV induction.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24119752