Significantly different results in the ocular surface microbiome detected by tear paper and conjunctival swab

Great variation has been observed in the composition of the normal microbiota of the ocular surface, and therefore, in addition to differences in detection techniques, the method of collecting ocular surface specimens has a significant impact on the test results.The goal of this study is to ascertai...

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Bibliographic Details
Published in:BMC microbiology 2023-01, Vol.23 (1), p.31-31, Article 31
Main Authors: Chen, Zhangling, Xiang, Zhaoyu, Cui, Lipu, Qin, Xinran, Chen, Shuli, Jin, Huiyi, Zou, Haidong
Format: Article
Language:English
Subjects:
16S rRNA
Adult
Aged
Bacteria
Comparative analysis
Composition
Conjunctiva
Data analysis
Diabetes
Diabetes Mellitus
Eye
Eye diseases
Genetic testing
Health aspects
Humans
Identification and classification
Medical testing products
Methods
Microbes
Microbial activity
Microbiomes
Microbiota
Microbiota (Symbiotic organisms)
Microorganisms
Middle Aged
Next-generation sequencing
Ocular surface
Older people
Patients
RNA, Ribosomal, 16S - genetics
rRNA 16S
Sample size
Sampling
Sampling methods
Statistical analysis
Strip
Tear tests
Tears
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Summary:Great variation has been observed in the composition of the normal microbiota of the ocular surface, and therefore, in addition to differences in detection techniques, the method of collecting ocular surface specimens has a significant impact on the test results.The goal of this study is to ascertain whether the eye surface microbial communities detected by two different sampling methods are consistent and hence explore the feasibility of using tear test paper instead of conjunctival swabs to collect eye surface samples for microbial investigation. From July 15, 2021, to July 30, 2021, nonirritating tear test strips and conjunctival swabs of both eyes were used in 158 elderly people (> 60 years old) (79 diabetic and 79 nondiabetic adults) in Xinjing Community for high-throughput sequencing of the V3-V4 region of the 16S rRNA gene. The composition of the microbial communities in tear test paper and conjunctival swab samples was analyzed. There was no statistically significant difference in Alpha diversity of ocular surface microorganisms represented by tear strip and conjunctival swab in diabetic group (P > 0.05), but there was statistically significant difference in Alpha diversity of ocular surface microorganisms detected by tear strip and conjunctival swab in nondiabetic group (P 
ISSN:1471-2180
1471-2180
DOI:10.1186/s12866-023-02775-3