Is there a Function for a Sex Pheromone Precursor?

Functional coupling and comparative genomics analysis have been applied to study functional associations of orthologs of enterococcal cAD1 sex pheromone (P13268) known to be responsible for biofilm formation, conjugative plasmid transfer and spreading of bacterial antibiotics resistance. cAD1 peptid...

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Bibliographic Details
Published in:Journal of integrative bioinformatics 2019-07, Vol.16 (4), p.777-97
Main Authors: Vasieva, O., Goryanin, I.
Format: Article
Language:English
Subjects:
Amino Acid Motifs
ApbE
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
cAD1
Enterobacteriaceae - genetics
Enterobacteriaceae - metabolism
enterococcus
Lipoproteins - genetics
Lipoproteins - metabolism
Membrane Proteins - genetics
Membrane Proteins - metabolism
Molecular Chaperones - genetics
Molecular Chaperones - metabolism
Oligopeptides - genetics
Oligopeptides - metabolism
Protein Processing, Post-Translational - physiology
redox metabolism
SEED
Treponema - genetics
Treponema - metabolism
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Summary:Functional coupling and comparative genomics analysis have been applied to study functional associations of orthologs of enterococcal cAD1 sex pheromone (P13268) known to be responsible for biofilm formation, conjugative plasmid transfer and spreading of bacterial antibiotics resistance. cAD1 peptide pheromone is released from the membrane lipoprotein with the peptide precursor encoded by a gene (tr|C2JQE7). Our analysis of genomic neighbourhood of and motifs of the encoded polypeptide and its orthologs suggests a close functional association between cAD1 and ApbE protein (Q82Z24), a FMN insertion and trafficking facilitator. The and orthologs were coupled in the genomes and ApbE-specific motifs for FMN covalent attachment were identified in -encoded protein sequence and its orthologs. These findings suggest a potential role of FMN-based reductase function of the cAD1 lipoprotein precursor in its processing and release of the active sex pheromone peptide. They may lead to a new approach in prevention of antibiotic resistance spread via targeting sex pheromone processing chaperones or by suppression of the FMN availability and covalent binding. This methods can be also applied to a controlled evolution of bacterial pathogenicity in microbial fuel cells, as the findings suggest the crosstalk between bacterial pathogenicity and bacterial electro-activity.
ISSN:1613-4516
DOI:10.1515/jib-2019-0016