The Application of Adipose Tissue-Derived Mesenchymal Stem Cells (ADMSCs) and a Twin-Herb Formula to the Rodent Wound Healing Model: Use Alone or Together?

• Published in: International journal of molecular sciences 2023-01, Vol.24 (2), p.1372
• Main Authors: Ma, Hui, Siu, Wing-Sum, Koon, Chi-Man, Wu, Xiao-Xiao, Li, Xiang, Cheng, Wen, Shum, Wai-Ting, Lau, Clara Bik-San, Wong, Chun-Kwok, Leung, Ping-Chung
• Format: Article
• Language: English
• Subjects: Adipose tissue; Angiogenesis; Animals; Anti-Inflammatory Agents - pharmacology; Apoptosis; Body fat; Cell viability; Chinese medicine; Collagen; Collagen (type I); Collagen (type III); Collagen - pharmacology; Cytokines - pharmacology; Diabetes; Drugs, Chinese Herbal - pharmacology; Extracellular matrix; Herbs; Infections; Inflammation; Interleukin 10; Interleukin 6; Mesenchymal Stem Cell Transplantation; Mesenchymal Stem Cells; MSCs; NF3; Proteins; Proteomics; Rodentia; Smad protein; Sox9 protein; Stem cell transplantation; Stem cells; Transforming Growth Factor beta - pharmacology; Tumor necrosis factor-α; Ulcers; Wnt protein; Wound Healing
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24021372

Our previous study reported that mesenchymal stem cells (MSCs) accelerated the wound healing process through anti-inflammatory, anti-apoptotic, and pro-angiogenetic effects in a rodent skin excision model. NF3 is a twin-herb formula, which presents similar effects in promoting wound healing. Research focusing on the interaction of MSCs and Chinese medicine is limited. In this study, we applied MSCs and the twin-herb formula to the wound healing model and investigated their interactions. Wound healing was improved in all treatment groups (MSCs only, NF3 only, and MSCs + NF3). The combined therapy further enhanced the effect: more GFP-labelled ADMSCs, collagen I and collagen III expression, Sox9 positive cells, and CD31 positive cells, along with less ED-1 positive cells, were detected; the expressions of proinflammatory cytokine IL-6 and TNF-α were downregulated; and the expression of anti-inflammatory cytokine IL-10 was upregulated. In vitro, NF3 promoted the cell viability and proliferation ability of MSCs, and a higher concentration of protein was detected in the NF3-treated supernatant. A proteomic analysis showed there were 15 and 22 proteins in the supernatants of normal ADMSCs and NF3-treated ADMSCs, respectively. After PCR validation, the expressions of 11 related genes were upregulated. The results of a western blot suggested that the TGFβ/Smad and Wnt pathways were related to the therapeutic effects of the combined treatment. Our study suggests for the first time that NF3 enhanced the therapeutic effect of MSCs in the wound healing model and the TGFβ/Smad and Wnt pathways were related to the procedure.