Development and validation of an ELISA using a protein encoded by ORF2 antigenic domain of porcine circovirus type 2

The capsid protein (ORF2) is a major structural protein of porcine circovirus type 2 (PCV2). A simple and reliable diagnostic method based on ORF2 protein immunoreactivity would serve as a valuable diagnostic method for detecting serum antibodies to PCV2 and monitoring PCV infection. Here, we report...

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Bibliographic Details
Published in:Virology journal 2010-10, Vol.7 (1), p.274-274, Article 274
Main Authors: Sun, Shi-Qi, Guo, Hui-Chen, Sun, De-Hui, Yin, Shuang-Hui, Shang, You-Jun, Cai, Xue-Peng, Liu, Xiang-Tao
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - blood
Antigens, Viral
Capsid Proteins
Circoviridae Infections - diagnosis
Circoviridae Infections - veterinary
Circovirus - immunology
Circovirus - isolation & purification
Cloning
Deoxyribonucleic acid
Diagnosis
DNA
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Escherichia coli
Escherichia coli - genetics
Genetic aspects
Genomes
Hogs
Milk
Physiological aspects
Porcine circovirus
Recombinant Proteins
Sensitivity and Specificity
Standard deviation
Swine
Swine Diseases - diagnosis
Swine Diseases - virology
Technological change
Viral proteins
Virology - methods
Virus diseases
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Summary:The capsid protein (ORF2) is a major structural protein of porcine circovirus type 2 (PCV2). A simple and reliable diagnostic method based on ORF2 protein immunoreactivity would serve as a valuable diagnostic method for detecting serum antibodies to PCV2 and monitoring PCV infection. Here, we reported an indirect enzyme-linked immunosorbent assay (I-ELISA) by using an antigenic domain (113-147AA) of ORF2-encoded antigen, expressed in E. coli, for diagnosis of PCV infection. The ELISA was performed on 288 serum samples collected from different porcine herds and compared with an indirect immunofluorescent assay (IFA). In total, 262 of 288 samples were positive as indicated by both I-ELISA and IFA. The specificity and sensitivity of I-ELISA were 87.7% and 93.57%. This ELISA is suitable for detection and discrimination of PCV2 infection in both SPF and farm antisera.
ISSN:1743-422X
1743-422X
DOI:10.1186/1743-422X-7-274