Cytotoxicity of intracanal dressings on apical papilla cells differ upon activation with E. faecalis LTA

The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC). Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste...

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Bibliographic Details
Published in:Journal of applied oral science 2019-01, Vol.27, p.e20180291-e20180291
Main Authors: Sipert, Carla Renata, Oliveira, Aline Pereira, Caldeira, Celso Luiz
Format: Article
Language:English
Subjects:
Adolescent
Analysis of Variance
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - toxicity
Calcium Hydroxide - chemistry
Calcium Hydroxide - toxicity
Cefaclor - chemistry
Cefaclor - toxicity
Cell culture techniques
Cell Survival - drug effects
Cells, Cultured
Ciprofloxacin - chemistry
Ciprofloxacin - toxicity
Dental Papilla - cytology
Dental Papilla - drug effects
Dental pulp cavity
Dentistry
DENTISTRY, ORAL SURGERY & MEDICINE
Enterococcus faecalis - chemistry
Female
Humans
Immunity, Cellular
Lipopolysaccharides - toxicity
Male
Metronidazole - chemistry
Metronidazole - toxicity
Original
Reproducibility of Results
Root Canal Irrigants - toxicity
Teichoic Acids - toxicity
Time Factors
Tooth Apex - cytology
Tooth Apex - drug effects
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Summary:The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC). Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay. The substances were reconstituted in DMEM at 1,000 µg/mL and ¼ serially diluted before being kept in contact with cells for 1, 3, 5 and 7 days. Further, cells were primed with 1 µg/mL of Enterococcus faecalis LTA for 7 days prior to the viability test with 1,000 µg/mL of each substance. Statistical analysis was performed using one-way analysis of variance (ANOVA) and two-way ANOVA respectively followed by Tukey's post-test. Significance levels were set at p
ISSN:1678-7757
1678-7765
1678-7765
DOI:10.1590/1678-7757-2018-0291