Stem Cell Therapy Enhances Motor Activity of Triceps Surae Muscle in Mice with Hereditary Peripheral Neuropathy

• Published in: International journal of molecular sciences 2021-11, Vol.22 (21), p.12026
• Main Authors: Govbakh, Iryna, Kyryk, Vitalii, Ustymenko, Alina, Rubtsov, Volodymyr, Tsupykov, Oleg, Bulgakova, Nataliya V, Zavodovskiy, Danylo O, Sokolowska, Inna, Maznychenko, Andriy
• Format: Article
• Language: English
• Subjects: Animals; Approximation; Cell therapy; Cell- and Tissue-Based Therapy - methods; Charcot-Marie-Tooth disease; Charcot-Marie-Tooth Disease - genetics; Charcot-Marie-Tooth Disease - metabolism; Charcot-Marie-Tooth Disease - physiopathology; Charcot-Marie-Tooth Disease - therapy; Chemical compounds; Cytokines; Disease Models, Animal; Electric Stimulation - methods; electrical stimulation; Electrical stimuli; Force; Growth factors; hereditary peripheral neuropathy; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - metabolism; Mesenchyme; Mice; Mice, Inbred C57BL; Mice, Transgenic; Motor activity; Motor Activity - physiology; Muscle Contraction; Muscle strength; Muscle, Skeletal - physiology; Muscular function; Nerve Regeneration - physiology; Neurological disorders; Peripheral neuropathy; Pharmacology; Regeneration; Sciatic nerve; Stem cells; Stromal cells; Transplantation; Transplants & implants; Triceps surae muscle
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms222112026

Impaired motor and sensory functions are the main features of Charcot-Marie-Tooth disease. Mesenchymal stem cell (MSCs) therapy is one of the possible treatments for this disease. It was assumed that MSCs therapy can improve the contractile properties of the triceps surae (TS) muscles in mice with hereditary peripheral neuropathy. Murine adipose-derived mesenchymal stromal cells (AD-MSCs) were obtained for transplantation into TS muscles of mice. Three months after AD-MSCs transplantation, animals were subjected to electrophysiological investigations. Parameters of TS muscle tension after intermittent high frequency electrical sciatic nerve stimulations were analyzed. It was found that force of TS muscle tension contraction in animals after AD-MSCs treatment was two-time higher than in untreated mice. Normalized values of force muscle contraction in different phases of electrical stimulation were 0.3 ± 0.01 vs. 0.18 ± 0.01 and 0.26 ± 0.03 vs. 0.13 ± 0.03 for treated and untreated animals, respectively. It is assumed that the two-fold increase in TS muscle strength was caused by stem cell therapy. Apparently, AD-MSCs therapy can promote nerve regeneration and partial restoration of muscle function, and thus can be a potential therapeutic agent for the treatment of peripheral neuropathies.