Inoculation with Stutzerimonas stutzeri strains decreases N₂O emissions from vegetable soil by altering microbial community composition and diversity

• Published in: Microbiology spectrum 2024-05, Vol.12 (5), p.e0018624-e0018624
• Main Authors: Gao, Nan, Zhang, Huanhuan, Hu, Chun, Li, Qing, Li, Linmei, Lei, Peng, Xu, Hong, Shen, Weishou
• Format: Article
• Language: English
• Subjects: Agriculture - methods; Environmental Microbiology; microbial community composition; Microbiota; nitrogen-cycle functional genes; nitrous oxide; Nitrous Oxide - metabolism; plant growth-promoting rhizobacteria (PGPR); Pseudomonas stutzeri - genetics; Pseudomonas stutzeri - growth & development; Pseudomonas stutzeri - metabolism; Research Article; Soil - chemistry; Soil Microbiology; soil texture; Solanum lycopersicum - growth & development; Solanum lycopersicum - microbiology; Vegetables - growth & development; Vegetables - microbiology
• ISSN: 2165-0497; 2165-0497
• DOI: 10.1128/spectrum.00186-24

Inoculation with plant growth-promoting rhizobacteria (PGPR) strains has promoted plant growth and decreased nitrous oxide (N₂O) emissions from agricultural soils simultaneously. However, limited PGPR strains can mitigate N₂O emissions from agricultural soils, and the microbial ecological mechanisms underlying N₂O mitigation after inoculation are poorly understood. In greenhouse pot experiments, the effects of inoculation with NRCB010 and NRCB025 on tomato growth and N₂O emissions were investigated in two vegetable agricultural soils with contrasting textures. Inoculation with NRCB010 and NRCB025 significantly promoted tomato growth in both soils. Moreover, inoculation with NRCB010 decreased the N₂O emissions from the fine- and coarse-textured soils by 38.7% and 52.2%, respectively, and inoculation with NRCB025 decreased the N₂O emissions from the coarse-textured soil by 76.6%. Inoculation with NRCB010 and NRCB025 decreased N₂O emissions mainly by altering soil microbial community composition and the abundance of nitrogen-cycle functional genes. The N₂O-mitigating effect might be partially explained by a decrease in the ( )/( I + II) and ( )/( I + II) ratios, respectively. Soil pH and organic matter were key variables that explain the variation in abundance of N-cycle functional genes and subsequent N₂O emission. Moreover, the N₂O-mitigating effect varied depending on soil textures and individual strain after inoculation. This study provides insights into developing biofertilizers with plant growth-promoting and N₂O-mitigating effects. Plant growth-promoting rhizobacteria (PGPR) have been applied to mitigate nitrous oxide (N₂O) emissions from agricultural soils, but the microbial ecological mechanisms underlying N₂O mitigation are poorly understood. That is why only limited PGPR strains can mitigate N₂O emissions from agricultural soils. Therefore, it is of substantial significance to reveal soil ecological mechanisms of PGPR strains to achieve efficient and reliable N₂O-mitigating effect after inoculation. Inoculation with strains decreased N₂O emissions from two soils with contrasting textures probably by altering soil microbial community composition and gene abundance involved in nitrification and denitrification. Our findings provide detailed insight into soil ecological mechanisms of PGPR strains to mitigate N₂O emissions from vegetable agricultural soils.