Total serum IgD from healthy and sick dogs with leishmaniosis

• Published in: Parasites & vectors 2019-03, Vol.12 (1), p.119-119, Article 119
• Main Authors: Martínez-Orellana, Pamela, Maristany, Cristina, Baxarias, Marta, Álvarez-Fernández, Alejandra, Baldassarre, Antonella, Ordeix, Laura, Solano-Gallego, Laia
• Format: Article
• Language: English
• Subjects: Analysis; Animals; Antibodies; Antibodies, Protozoan - blood; Antibodies, Protozoan - immunology; Antigens; B cells; Biological response modifiers; Blood serum; Canine leishmaniosis; Cell proliferation; Cell-mediated immunity; Cells; Concanavalin A; Demographics; Dog Diseases - blood; Dog Diseases - immunology; Dog Diseases - parasitology; Dogs; ELISA; Enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - veterinary; Exhaustion; Female; Health aspects; Human performance; IFN-γ; IgD; IL-10; Immune response; Immune response (humoral); Immunity; Immunoglobulin D; Immunoglobulin D - blood; Immunoglobulin D - immunology; Immunoglobulins; Infections; Interferon; Leishmania infantum; Leishmania infantum - immunology; Leishmaniasis; Leishmaniasis, Visceral - immunology; Leishmaniasis, Visceral - parasitology; Leishmaniasis, Visceral - veterinary; Lymphocytes; Lymphocytes B; Lymphocytes T; Male; Mitogens; Multiple myeloma; Parasites; Parasitic diseases; PD-1; Proliferation; Proteins; Retrospective Studies; Risk factors; Serum; Stimulation; T cells; Tropical diseases; γ-Interferon
• ISSN: 1756-3305; 1756-3305
• DOI: 10.1186/s13071-019-3384-0

Canine leishmaniosis (CanL) due to Leishmania infantum is characterized by the development of both cellular and humoral immune responses. The dysfunction of T cell-mediated immunity leads to a lack of proliferation of T cells in response to Leishmania antigens with the consequence of parasite dissemination that seems to be related to a T cell exhaustion mediated by regulatory B cells expressing immunoglobulin D (IgD). The aim of this study was to determine and compare the total serum IgD in dogs with clinical leishmaniosis and in clinically healthy dogs. A total of 147 dog sera were studied. All dogs were tested for L. infantum-specific antibodies by quantitative ELISA. Interferon-gamma (IFN-γ) production was also determined by sandwich ELISA after blood stimulation with L. infantum soluble antigen (LSA) or concanavalin A (ConA). The quantification of total IgD was performed using a human IgD sandwich ELISA quantification set. Dogs were classified in three different groups. Group 1 included 40 clinically healthy non-infected dogs, all serologically negative to L. infantum-specific antibodies and non-producers of IFN-γ upon LSA stimulation. Group 2 included 63 clinically healthy infected dogs that were LSA IFN-γ producers (n = 61) and/or IFN-γ non-producers (n = 2) as well as negative to medium seropositive to L. infantum antigen. Finally, Group 3 included 44 dogs with clinical leishmaniosis (IFN-γ producers, n = 23; and IFN-γ non-producers, n = 21) that were negative to highly positive to L. infantum-specific antibodies. No significant differences were observed when the total IgD concentration was compared within groups. Additionally, total IgD of sick IFN-γ producers and IFN-γ non-producers was not significantly different. Finally, total IgD concentration was not statistically related to demographic parameters such as age, sex and breed. The results of this study demonstrated that there were no differences between groups in total serum IgD. Total serum IgD does not appear to be a marker of disease in CanL.