Application of UV-Vis spectrophotometric and chemiluminescent methods for evaluation of the antioxidant action of curcumin

Curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5- dione) is a natural biological active substance with an antioxidant activity. The ability of curcumin to inhibit the free radical mechanisms can be used in a prevention of diseases such as cancer and coronary heart disease. The UV-VIS...

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Bibliographic Details
Published in:Journal of the Serbian Chemical Society 2012, Vol.77 (8), p.1063-1069
Main Authors: Stanchev, Stancho, Pencheva, Ivanka, Konstantinov, Spiro, Obreshkova, Danka, Hadjimitova, Vera
Format: Article
Language:English
Subjects:
antioxidant
chemiluminescence
curcumin
DNA complexation
UV-Vis spectrophotometry
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Summary:Curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5- dione) is a natural biological active substance with an antioxidant activity. The ability of curcumin to inhibit the free radical mechanisms can be used in a prevention of diseases such as cancer and coronary heart disease. The UV-VIS spectrophotometric and chemiluminescent dynamic methods for determination of antioxidant activity of curcumin were developed. The spectrophotometric method includes investigation of the interaction between DNA, isolated from HL-60 cells, and curcumin. The decreasing of the absorption of curcumin in the presence of HL-60 DNA against the blank sample can be a measurement for some complex formation between curcumin and DNA. The chemiluminescent method involves three tests for detection of luminol - depending chemiluminescence on the base of model systems which generate superoxide, hydroxide and hypochlorite radicals. The strongest decay of chemilunimescence was registered at the highest concentration of curcumin (100 ?mol/L). nema
ISSN:0352-5139
1820-7421
DOI:10.2298/JSC110621032S