Interleukin-5 mediates peritoneal eosinophilia induced by the F1 cell wall fraction of Histoplasma capsulatum

• Published in: Brazilian journal of medical and biological research 2004-03, Vol.37 (3), p.343-346
• Main Authors: Sá-Nunes, A, Medeiros, A I, Faccioli, L H
• Format: Article
• Language: English
• Subjects: Animals; Anti-IL-5 monoclonal antibody; Antibodies, Monoclonal - administration & dosage; Antibodies, Monoclonal - immunology; BIOLOGY; Cell Count; Cell Movement; Cell Wall - chemistry; Eosinophilia - etiology; Eosinophils; Eosinophils - drug effects; Eosinophils - physiology; Glucans - isolation & purification; Glucans - pharmacology; Histoplasma - chemistry; Histoplasma capsulatum; Inflammation; Interleukin-5; Interleukin-5 - physiology; Male; MEDICINE, RESEARCH & EXPERIMENTAL; Mice; Mice, Inbred C57BL; Peritoneal Cavity - cytology
• ISSN: 0100-879X; 1414-431X; 0100-879X; 1414-431X
• DOI: 10.1590/S0100-879X2004000300009

An alkali-insoluble fraction 1 (F1), which contains mainly ss-glucan isolated from the cell wall of Histoplasma capsulatum, induces eosinophil recruitment into the peritoneal cavity of mice. The present study was carried out to determine the participation of interleukin-5 (IL-5) in this process. Inbred C57BL/6 male mice weighing 15-20 g were treated ip with 100 microg of anti-IL-5 monoclonal antibody (TRFK-5, N=7) or an isotype-matched antibody (N=7), followed by 300 microg F1 in 1 ml PBS ip 24 h later. Controls (N=5) received only 1 ml PBS. Two days later, cells from the peritoneal cavity were harvested by injection of 3 ml PBS and total cell counts were determined using diluting fluid in a Neubauer chamber. Differential counts were performed using Rosenfeld-stained cytospin preparations. The F1 injection induced significant (P