Multiplex-PCR for detection of natural Leishmania infection in Lutzomyia spp. captured in an endemic region for cutaneous leishmaniasis in state of Sucre, Venezuela

We studied the natural infection of Lutzomyia (Lutzomyia) sp. with Leishmania in endemic foci of cutaneous leishmaniasis in the Paria peninsula, state of Sucre, Venezuela. Sand flies were collected between March 2001 and June 2003, using Shannon light-traps and human bait. Of the 1291 insects captur...

Full description

Saved in:
Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz 2005-02, Vol.100 (1), p.45-48
Main Authors: Jorquera, Alicia, González, Ricardo, Marchán-Marcano, Edgar, Oviedo, Milagros, Matos, Mercedes
Format: Article
Language:English
Subjects:
Animals
cutaneous leishmaniasis
cutaneous leishmaniasis - sand flies vectors - natural infections - polymerase chain reaction - Lutzomyia spp. - Venezuela
DNA, Protozoan - analysis
Female
Humans
Insect Vectors - parasitology
Leishmania
Leishmania - classification
Leishmania - isolation & purification
Leishmaniasis, Cutaneous - transmission
Lutzomyia gomezi
Lutzomyia spp
natural infections
PARASITOLOGY
polymerase chain reaction
Polymerase Chain Reaction - methods
Psychodidae - parasitology
sand flies vectors
TROPICAL MEDICINE
Venezuela
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We studied the natural infection of Lutzomyia (Lutzomyia) sp. with Leishmania in endemic foci of cutaneous leishmaniasis in the Paria peninsula, state of Sucre, Venezuela. Sand flies were collected between March 2001 and June 2003, using Shannon light-traps and human bait. Of the 1291 insects captured, only two species of phlebotomines were identified: L. ovallesi (82.75%) and L. gomezi (17.42%). A sample of the collected sand flies (51 pools of 2-12 individuals) were analyzed by using a multiplex-PCR assay for simultaneous detection of New Word Leishmania and Viannia subgenera. The results showed a total of 8 pools (15.68%) infected; of these, 7 were L. ovallesi naturally infected with L. braziliensis (2 pools) and L. mexicana (5 pools) and 1 pool of L. gomezi infected by L. braziliensis.
ISSN:1678-8060
0074-0276
0074-0276
1678-8060
DOI:10.1590/S0074-02762005000100008