Serotonin-mediated modulation of Na+/K+ pump current in rat hippocampal CA1 pyramidal neurons

• Published in: BMC neuroscience 2012-01, Vol.13 (1), p.10-10, Article 10
• Main Authors: Zhang, Li Nan, Su, Su Wen, Guo, Fang, Guo, Hui Cai, Shi, Xiao Lu, Li, Wen Ya, Liu, Xu, Wang, Yong Li
• Format: Article
• Language: English
• Subjects: Animals; Animals, Newborn; Biguanides - pharmacology; Biophysics; CA1 Region, Hippocampal - cytology; Dose-Response Relationship, Drug; Electric Stimulation; In Vitro Techniques; Neural Inhibition - drug effects; Neurons; Patch-Clamp Techniques; Piperazines - pharmacology; Pyramidal Cells - drug effects; Pyramidal Cells - physiology; Pyridines - pharmacology; Rats; Rats, Sprague-Dawley; Rodents; Serotonin - pharmacology; Serotonin Antagonists - pharmacology; Serotonin Receptor Agonists - pharmacology; Sodium Channel Blockers - pharmacology; Sodium-Potassium-Exchanging ATPase - metabolism; Strophanthidin - pharmacology; Studies; Tetrodotoxin - pharmacology
• ISSN: 1471-2202; 1471-2202
• DOI: 10.1186/1471-2202-13-10

The aim of this study was to investigate whether serotonin (5-hydroxytryptamine, 5-HT) can modulate Na+/K+ pump in rat hippocampal CA1 pyramidal neurons. 5-HT (0.1, 1 mM) showed Na+/K+ pump current (Ip) densities of 0.40 ± 0.04, 0.34 ± 0.03 pA/pF contrast to 0.63 ± 0.04 pA/pF of the control of 0.5 mM strophanthidin (Str), demonstrating 5-HT-induced inhibition of Ip in a dose-dependent manner in hippocampal CA1 pyramidal neurons. The effect was partly attenuated by ondasetron, a 5-HT3 receptor (5-HT3R) antagonist, not by WAY100635, a 5-HT1AR antagonist, while 1-(3-Chlorophenyl) biguanide hydrochloride (m-CPBG), a 5-HT3R specific agonist, mimicked the effect of 5-HT on Ip. 5-HT inhibits neuronal Na+/K+ pump activity via 5-HT3R in rat hippocampal CA1 pyramidal neurons. This discloses novel mechanisms for the function of 5-HT in learning and memory, which may be a useful target to benefit these patients with cognitive disorder.