Overview of distinct 5-methylcytosine profiles of messenger RNA in human hepatocellular carcinoma and paired adjacent non-tumor tissues

Post-transcriptional methylation modifications, including 5-methylcytosine (m5C) modification, are closely related to the tumorigenesis of cancers. However, the mRNA profile of m5C modification in hepatocellular carcinoma (HCC) is unknown. Methylated RNA immunoprecipitation sequencing was performed...

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Bibliographic Details
Published in:Journal of translational medicine 2020-06, Vol.18 (1), p.245-245, Article 245
Main Authors: Zhang, Qiyao, Zheng, Qingyuan, Yu, Xiao, He, Yuting, Guo, Wenzhi
Format: Article
Language:English
Subjects:
5-Methylcytosine
Analysis
Bioinformatics
Biomarkers
Carcinoma, Hepatocellular - genetics
Development and progression
DNA methylation
Genes
Genomes
Genomics
Hepatocellular carcinoma
Humans
Immunoprecipitation
Liver cancer
Liver Neoplasms - genetics
MeRIP-seq
Messenger RNA
Methylation
Methyltransferases
mRNA
Pathogenesis
Post-transcription
Proteins
Pyrimidines
Quality control
Researchers
RNA methylation
RNA modification
RNA, Messenger - genetics
Sequence Analysis, RNA
Stem cells
Transcription (Genetics)
Tumorigenesis
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Summary:Post-transcriptional methylation modifications, including 5-methylcytosine (m5C) modification, are closely related to the tumorigenesis of cancers. However, the mRNA profile of m5C modification in hepatocellular carcinoma (HCC) is unknown. Methylated RNA immunoprecipitation sequencing was performed to identify m5C peaks on mRNA of human HCC tissues and adjacent tissues, and differences in m5C between the two groups were analyzed. In addition, we conducted a bioinformatics analysis to predict the function of specific methylated transcripts. We found that there was a noticeable difference in m5C between HCC and paired non-tumor tissues, suggesting that m5C could play a role in the pathogenesis of HCC. In addition, analyses of gene ontology and the Kyoto Encyclopedia of Genes and Genomes showed that the unique distribution pattern of mRNA m5C in HCC was associated with a wide range of cellular functions. Our results revealed different distribution patterns of m5C in HCC and adjacent tissues and provided new insights into a novel function of m5C RNA methylation of mRNA in HCC progression.
ISSN:1479-5876
1479-5876
DOI:10.1186/s12967-020-02417-6