Decreased inflammatory gene expression accompanies the improvement of liver enzyme and lipid profile following aerobic training and vitamin D supplementation in T2DM patients

• Published in: BMC endocrine disorders 2022-10, Vol.22 (1), p.1-245, Article 245
• Main Authors: Hoseini, Rastegar, Rahim, Hiwa Ahmed, Ahmed, Jalal Khdhr
• Format: Article
• Language: English
• Subjects: Aerobics; Alfacalcidol; Analysis; Biological response modifiers; Biomarkers; Body mass index; Bone morphogenetic proteins; Calcifediol; CD27 antigen; Chemokines; Chronic illnesses; Clinical trials; CXCL13 protein; Cytokines; Diabetes; Diabetes mellitus (non-insulin dependent); Dietary supplements; Enzymes; Exercise; Gene expression; Genes; Glucose; Health aspects; High density lipoprotein; Inflammation; Insulin resistance; Interferon; Interleukin 10; Interleukin 6; Interleukins; Leukocytes (mononuclear); Lipids; Liver; Low density lipoprotein; Low density lipoproteins; Medical research; Medicine, Experimental; Neuromuscular diseases; Peripheral blood mononuclear cells; Pharmaceutical industry; Physical fitness; Placebos; Serum levels; Supplements; Transforming growth factor-b1; Transforming growth factors; Type 2 diabetes; Variance analysis; Vitamin D; Vitamin deficiency; γ-Interferon
• ISSN: 1472-6823; 1472-6823
• DOI: 10.1186/s12902-022-01152-x

Background Type 2 Diabetes Mellitus (T2DM) is one of the health issues causing untoward low-grade systemic inflammation. Aerobic Training (AT) and Vitamin D (Vit D) supplementation are among the approaches that improve lipid profile and liver enzymes in T2DM. However, the mechanisms responsible for these improvements are not fully elucidated. Objectives This study aimed to evaluate the effects of AT and Vit D supplementation on lipid profile, liver enzymes, Interleukin-6 (IL-6), Interleukin-10 (IL-10), Cluster of differentiation 27 (CD27), Chemokine (C-X-C motif) Ligand 13 (CXCL13), Interferon-Gamma (IFN-[gamma]) and Transforming Growth Factor-Beta 1 (TGF-[beta]1) gene expressions in patients with T2DM. Methods In this study, 40 male T2DM patients aged 35-50 years were randomly selected and assigned into four groups (n = 10 for each); AT+vitamin D supplementation (AT+Vit D), AT+placebo (AT), Vit D supplementation (Vit D), and control+placebo (C). The intervention consisted of 8 weeks of 20-40 minutes AT protocol at 60-75% HR.sub.max 3 sessions/week and taking 50,000 IU of Vit D supplement once a week. Serum levels of lipid profile and liver enzymes and gene expression of IL-6, IL-10, CD27, CXCL13, IFN-[gamma], and TGF-[beta]1 in Peripheral Blood Mononuclear Cells (PBMCs) were measured. One-way analysis of variance (ANOVA), Tukey's post hoc, and paired sample t-test at P-values less than 0.05 were used to analyze the data using SPSS software. Results AT+Vit D, AT, and Vit D significantly decreased TC, TG, LDL, AST, ALT, and GGT while increased HDL after 8 weeks in favor of AT+Vit D. Also, gene expressions of IL-6, IL-10, CD27, CXCL13, IFN-[gamma], and TGF-[beta]1 were downregulated significantly in AT+Vit D, AT, and Vit D, while upregulated in C. Furthermore, compared to individual AT or Vit D, AT+Vit D significantly downregulated IL-6 (P = 0.013; P = 0.025), IL-10 (P = 0.012; P = 0.026), CD27 (P = 0.023; P = 0.041), CXCL13 (P = 0.014; P = 0.025), IFN-[gamma] (P = 0.017; P = 0.026), and TGF-[beta]1 (P = 0.001; P = 0.028). Conclusion In comparison to individual AT or Vit D, AT+Vit D may enhance lipid profile, and liver enzymes and drive the balance to favor inhibition of inflammation by downregulating gene expression of inflammation-related factors. As a result, AT+Vit D may be considered appropriate therapy for managing T2DM. Keywords: Exercise, Inflammation, Diabetes, Vitamin D