Enhanced Proliferation and Differentiation of Human Mesenchymal Stem Cell-laden Recycled Fish Gelatin/Strontium Substitution Calcium Silicate 3D Scaffolds

• Published in: Applied sciences 2020-03, Vol.10 (6), p.2168
• Main Authors: Yu, Chun-Ta, Wang, Fu-Ming, Liu, Yen-Ting, Lee, Alvin Kai-Xing, Lin, Tsung-Li, Chen, Yi-Wen
• Format: Article
• Language: English
• Subjects: 3-D printers; Alkaline phosphatase; Biocompatibility; Biodegradation; Biomedical materials; bioprinting; bone regeneration; Bones; Calcium; Calcium phosphates; Calcium silicates; Cell differentiation; cell-laden scaffold; Crosslinking; Design; Differentiation; Fabrication; fish gelatin methacrylate; Fourier transforms; Gelatin; Growth factors; Mechanical properties; Mesenchymal stem cells; Organs; Osteogenesis; Polymers; Recycling; recycling material; Regenerative medicine; Researchers; Scaffolds; Stem cells; Strontium; strontium-doped calcium silicate; Tensile strength; Therapeutic applications; Tissue engineering
• ISSN: 2076-3417; 2076-3417
• DOI: 10.3390/app10062168

Cell-encapsulated bioscaffold is a promising and novel method to allow fabrication of live functional organs for tissue engineering and regenerative medicine. However, traditional fabrication methods of 3D scaffolds and cell-laden hydrogels still face many difficulties and challenges. This study uses a newer 3D fabrication technique and the concept of recycling of an unutilized resource to fabricate a novel scaffold for bone tissue engineering. In this study, fish-extracted gelatin was incorporated with bioactive ceramic for bone tissue engineering, and with this we successfully fabricated a novel fish gelatin methacrylate (FG) polymer hydrogel mixed with strontium-doped calcium silicate powder (FGSr) 3D scaffold via photo-crosslinking. Our results indicated that the tensile strength of FGSr was almost 2.5-fold higher as compared to FG thus making it a better candidate for future clinical applications. The in-vitro assays illustrated that the FGSr scaffolds showed good biocompatibility with human Wharton jelly-derived mesenchymal stem cells (WJMSC), as well as enhancing the osteogenesis differentiation of WJMSC. The WJMSC-laden FGSr 3D scaffolds expressed a higher degree of alkaline phosphatase activity than those on cell-laden FG 3D scaffolds and this result was further proven with the subsequent calcium deposition results. Therefore, these results showed that 3D-printed cell-laden FGSr scaffolds had enhanced mechanical property and osteogenic-related behavior that made for a more suitable candidate for future clinical applications.