Occurrence, Genetic Variability of Tomato Yellow Ring Orthotospovirus Population and the Development of Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Its Rapid Detection

Tomato-infecting viruses have been considered as a serious threat to tomato crops in Poland. Therefore, during 2014–2021, 234 tomato samples delivered directly by greenhouse tomato growers to Plant Disease Clinic of IPP-NRI were tested. Eight virus species: pepino mosaic virus (PepMV), tomato yellow...

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Bibliographic Details
Published in:Viruses 2022-06, Vol.14 (7), p.1405
Main Authors: Zarzy?ska-Nowak, Aleksandra, Budzy?ska, Daria, Taberska, Agnieszka, J?drzejczak, Norbert, Minicka, Julia, Borodynko-Filas, Natasza, Hasiów-Jaroszewska, Beata
Format: Article
Language:English
Subjects:
Amino acid sequence
diagnostics
Diseases and pests
Evolution
evolutionary dynamics
Flowers & plants
Fruits
Gene amplification
Genetic analysis
Genetic aspects
Genetic variability
Greenhouses
Identification and classification
Infections
Methods
Mixed infection
Nucleocapsids
Plant diseases
Plant virus diseases
Plant viruses
Population
Population genetics
Proteins
Reverse transcription
RNA polymerase
RT-LAMP
tomato viruses
Tomatoes
TYRV
Virus diseases of plants
Viruses
Wilt
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Summary:Tomato-infecting viruses have been considered as a serious threat to tomato crops in Poland. Therefore, during 2014–2021, 234 tomato samples delivered directly by greenhouse tomato growers to Plant Disease Clinic of IPP-NRI were tested. Eight virus species: pepino mosaic virus (PepMV), tomato yellow ring orthotospovirus (TYRV), tomato spotted wilt orthotospovirus (TSWV), potato virus Y (PVY), cucumber mosaic virus (CMV), tomato black ring virus (TBRV) and tomato mosaic virus (ToMV) were detected in single or mixed infection in 89 samples. The presence of TYRV was established for the first time in Poland in 2014. Since then, its presence has been observed in single and mixed infection with TSWV and CMV. Here, we analysed the genetic variability of TYRV population based on complete nucleocapsid (N) protein gene sequence of 55 TYRV isolates. Maximum-likelihood reconstruction revealed the presence of three distinct, well-supported phylogroups. Moreover, the effect of host species on virus diversity was confirmed. Therefore, RT-LAMP assay was developed for the rapid and efficient detection of TYRV isolates that can be implemented in field and greenhouse conditions.
ISSN:1999-4915
1999-4915
DOI:10.3390/v14071405