Circular RNA SIPA1L1 regulates osteoblastic differentiation of stem cells from apical papilla via miR-204-5p/ALPL pathway

Osteogenesis is a complex biological process which requires the coordination of multiple molecular mechanisms. This research aimed to explore the biological role and underlying regulatory mechanism of circSIPA1L1 during the osteogenic differentiation of stem cells from apical papilla (SCAPs). EdU re...

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Bibliographic Details
Published in:Stem cell research & therapy 2020-11, Vol.11 (1), p.461-461, Article 461
Main Authors: Li, Yuzhi, Bian, Minxia, Zhou, Zhou, Wu, Xiao, Ge, Xingyun, Xiao, Tong, Yu, Jinhua
Format: Article
Language:English
Subjects:
Alkaline Phosphatase
ALPL
Cell Differentiation
Cell growth
Cells, Cultured
Cholecystokinin
circRNAs
Circular RNA
Dental Papilla - cytology
Dental pulp
Differentiation
Flow cytometry
Fluorescence in situ hybridization
Genes
GTPase-Activating Proteins
Humans
In Situ Hybridization, Fluorescence
Localization
MicroRNA
MicroRNAs
MicroRNAs - genetics
Mineralization
miR-204-5p
miRNA
Molecular modelling
Osteoblastogenesis
Osteoblasts
Osteogenesis
Osteogenesis - genetics
Phosphatase
Phosphatases
Physiology
Proteins
RNA, Circular - genetics
SCAPs
Software
Stem cells
Stem Cells - cytology
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Summary:Osteogenesis is a complex biological process which requires the coordination of multiple molecular mechanisms. This research aimed to explore the biological role and underlying regulatory mechanism of circSIPA1L1 during the osteogenic differentiation of stem cells from apical papilla (SCAPs). EdU retention assay, flow cytometry assay, and CCK-8 assay were used to evaluate the proliferation capacity of SCAPs. Western blot assay, alkaline phosphatase (ALP), and alizarin red staining (ARS) were conducted to investigate the biological roles of circSIPA1L1 and miR-204-5p. Fluorescence in situ hybridization was applied for circSIPA1L1 localization. Dual-luciferase reporter assay was performed to prove the interaction of circSIPA1L1 and miR-204-5p. CircSIPA1L1 had no significant effect on the proliferative capacity of SCAPs. CircSIPA1L1 promotes osteogenic differentiation of SCAPs by serving as a miRNA sponge for miR-204-5p. Either knockdown of circSIPA1L1 or overexpression of miR-204-5p significantly suppresses osteogenic differentiation of SCAPs. CircSIPA1L1 upregulates ALPL through targeting miR-204-5p and promotes the osteogenic differentiation of SCAPs.
ISSN:1757-6512
1757-6512
DOI:10.1186/s13287-020-01970-7