Leptospira interrogans enters different host cells by binding to distinct molecules in the extracellular matrix

Leptospirosis caused by Leptospira is a worldwide zoonotic disease. The 2 spirochete can efficiently invade host cells and spread from blood into internal organs, but the mechanisms of leptospiral internalization into different host cells remain poorly understood. Adhesion of leptospires to the ECM...

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Bibliographic Details
Published in:Archives of biological sciences 2015, Vol.67 (1), p.31-39
Main Authors: Zhao, Xin, Sun, Ai-Hua, Ge, Yu-Mei, Wang, Huan, Yan, Jie
Format: Article
Language:English
Subjects:
adhesion
extracellular matrix
internalization
Leptospira interrogans
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Summary:Leptospirosis caused by Leptospira is a worldwide zoonotic disease. The 2 spirochete can efficiently invade host cells and spread from blood into internal organs, but the mechanisms of leptospiral internalization into different host cells remain poorly understood. Adhesion of leptospires to the ECM (extracellular matrix) initiated leptospire internalization in macrophage cell-lines THP-1, J774A.1, and in human umbilical epithelial cells (HUVEC). We assessed the distribution of ECM , including fibronectin (FN), laminin and collagens 1-4 (COL1-COL4) in different cells. FN was expressed in THP-1 and J774A.1 cells, and FN, LN, COL3 and COL4 were expressed in HUVEC. Then, we found leptospires adhered to FN, LN, COL3 and COL4. The most important intracellular leptospires in macrophages (THP-1 and J774A) significantly decreased when leptospires were pre-blocked with FN This indicates that FN has an important role in the adherence to and entry of leptospires in macrophages, while in HUVEC, FN, LN, COL3 and COL4 assume this role.
ISSN:0354-4664
1821-4339
DOI:10.2298/ABS140702003Z