Tangential Flow Filtration for Highly Efficient Concentration of Extracellular Vesicles from Large Volumes of Fluid

Concentration of extracellular vesicles (EVs) from biological fluids in a scalable and reproducible manner represents a major challenge. This study reports the use of tangential flow filtration (TFF) for the highly efficient isolation of EVs from large volumes of samples. When compared to ultracentr...

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Bibliographic Details
Published in:Cells (Basel, Switzerland) Switzerland), 2018-12, Vol.7 (12), p.273
Main Authors: Busatto, Sara, Vilanilam, George, Ticer, Taylor, Lin, Wen-Lang, Dickson, Dennis W, Shapiro, Shane, Bergese, Paolo, Wolfram, Joy
Format: Article
Language:English
Subjects:
Adipose tissue
Antibodies
Brain research
Cell culture
Chromatography
Cloning
Communication
Culture media
exosome
Extracellular vesicles
Filtration
isolation
lipoaspirate
Macromolecules
Measles
Nanoparticles
Proteins
Sucrose
tangential flow filtration
Ultracentrifugation
ultrafiltration
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Summary:Concentration of extracellular vesicles (EVs) from biological fluids in a scalable and reproducible manner represents a major challenge. This study reports the use of tangential flow filtration (TFF) for the highly efficient isolation of EVs from large volumes of samples. When compared to ultracentrifugation (UC), which is the most widely used method to concentrate EVs, TFF is a more efficient, scalable, and gentler method. Comparative assessment of TFF and UC of conditioned cell culture media revealed that the former concentrates EVs of comparable physicochemical characteristics, but with higher yield, less single macromolecules and aggregates (
ISSN:2073-4409
2073-4409
DOI:10.3390/cells7120273