Unraveling the genomic secrets of Tritonibacter mobilis AK171: a plant growth-promoting bacterium isolated from Avicennia marina

• Published in: BMC genomics 2024-07, Vol.25 (1), p.672-17, Article 672
• Main Authors: Alghamdi, Amal Khalaf, Parween, Sabiha, Hirt, Heribert, Saad, Maged M
• Format: Article
• Language: English
• Subjects: Abiotic stress; Agriculture; Arabidopsis thaliana; Avicennia - microbiology; Bacteria; Biocontrol; Biofilms; Biosynthesis; Cell migration; Environmental aspects; Enzymes; Freshwater resources; Genes; Genetic analysis; Genetic aspects; Genetic diversity; Genome, Bacterial; Genomes; Genomic analysis; Genomics; Gram-negative bacteria; High temperature; Host plants; Living conditions; Mangrove; Mangrove plants; Mangroves; Metabolites; Microbiological research; Microorganisms; Nucleotide sequence; Phylogeny; Physiological aspects; Plant Development; Plant growth; Qualitative analysis; Red sea; Rhizosphere; Rosette; Saline water; Salinity; Salinity effects; Salt; Seeds; Sequence analysis; Siderophores; Siderophores - metabolism; Sustainable agriculture; Transcription activation; Waterlogging
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-024-10555-0

The scarcity of freshwater resources resulting in a significant yield loss presents a pressing challenge in agriculture. To address this issue, utilizing abundantly available saline water could offer a smart solution. In this study, we demonstrate that the genome sequence rhizosphere bacterium Tritonibacter mobilis AK171, a halophilic marine bacterium recognized for its ability to thrive in saline and waterlogged environments, isolated from mangroves, has the remarkable ability to enable plant growth using saline irrigation. AK171 is characterized as rod-shaped cells, displays agile movement in free-living conditions, and adopts a rosette arrangement in static media. Moreover, The qualitative evaluation of PGP traits showed that AK171 could produce siderophores and IAA but could not solubilize phosphate nor produce hydrolytic enzymes it exhibits a remarkable tolerance to high temperatures and salinity. In this study, we conducted a comprehensive genome sequence analysis of T. mobilis AK171 to unravel the genetic mechanisms underlying its plant growth-promoting abilities in such challenging conditions. Our analysis revealed diverse genes and pathways involved in the bacterium's adaptation to salinity and waterlogging stress. Notably, T. mobilis AK171 exhibited a high level of tolerance to salinity and waterlogging through the activation of stress-responsive genes and the production of specific enzymes and metabolites. Additionally, we identified genes associated with biofilm formation, indicating its potential role in establishing symbiotic relationships with host plants. Furthermore, our analysis unveiled the presence of genes responsible for synthesizing antimicrobial compounds, including tropodithietic acid (TDA), which can effectively control phytopathogens. This genomic insight into T. mobilis AK171 provides valuable information for understanding the molecular basis of plant-microbial interactions in saline and waterlogged environments. It offers potential applications for sustainable agriculture in challenging conditions.